Department of Basic Medical Sciences, Neuroscience and Sense Organs, University of Bari Aldo Moro, Bari, Italy.
Department of Medical and Surgical Sciences, Institute of Respiratory Diseases, University of Foggia, Policlinico "OO Riuniti", Foggia, Italy.
Arch Bronconeumol (Engl Ed). 2021 May;57(5):338-344. doi: 10.1016/j.arbres.2020.04.024. Epub 2020 Jun 24.
Asthma inflammation may feature an imbalance between oxidative stress and antioxidant defenses. Oxidative stress induces propagation of airways inflammation and corticosteroid insensitivity contributing to poor asthma control, and frequent severe acute exacerbations. This study assessed inflammation and oxidative stress in severe asthmatic subjects and evaluated the possible correlations between inflammatory and oxidative stress markers investigated and asthma severity.
Fifty-three patients with severe asthma, 11 patients with mild-moderate asthma and 12 healthy subjects were enrolled and underwent fractional exhaled nitric oxide (FENO) analysis and blood and sputum count cell collection. The content of mitochondrial DNA (MtDNA) and nuclear DNA (nDNA) was measured in exhaled breath condensate (EBC) by Real Time PCR and the ratio between MtDNA/nDNA was calculated. We detected MtDNA/nDNA in the EBC of severe asthmatics.
We found higher exhaled MtDNA/nDNA in severe asthmatics respectively compared to mild-moderate ones and to healthy controls (10.4±2.2 vs 7.9±2.5, p<0.05 and 10.4±2.2 vs 6.51±0.21, p<0.05). The level of exhaled MtDNA/nDNA was significantly higher in Non-T2 endotype severe asthmatics than T2 (14.07±10. 8 vs 6.5±5.5, p<0.05).
Oxidative stress marker (MtDNA/nDNA) is increased significantly with asthma severity and may be useful for endotyping severe asthma.
哮喘炎症可能表现为氧化应激和抗氧化防御之间的失衡。氧化应激诱导气道炎症的传播和皮质类固醇不敏感，导致哮喘控制不佳和频繁的严重急性加重。本研究评估了严重哮喘患者的炎症和氧化应激，并评估了所研究的炎症和氧化应激标志物与哮喘严重程度之间的可能相关性。
纳入 53 例重度哮喘患者、11 例轻中度哮喘患者和 12 例健康对照者，进行呼出气一氧化氮（FENO）分析及血痰细胞计数。采用实时 PCR 法测量呼出气冷凝液（EBC）中的线粒体 DNA（MtDNA）和核 DNA（nDNA）含量，并计算 MtDNA/nDNA 比值。我们检测了重度哮喘患者 EBC 中的 MtDNA/nDNA。
与轻中度哮喘患者和健康对照组相比，我们发现重度哮喘患者的呼出 MtDNA/nDNA 更高（10.4±2.2 比 7.9±2.5，p<0.05；10.4±2.2 比 6.51±0.21，p<0.05）。非 T2 表型重度哮喘患者的呼出 MtDNA/nDNA 水平明显高于 T2 表型（14.07±10.8 比 6.5±5.5，p<0.05）。
氧化应激标志物（MtDNA/nDNA）随哮喘严重程度显著增加，可能有助于对重度哮喘进行表型分型。
