Department of Material supply, the Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, Shandong, China.
Department of Cardiology, First Ward, Yantai Yeda Hospital, Yantai, Shandong, China.
Pharm Biol. 2023 Dec;61(1):449-458. doi: 10.1080/13880209.2023.2173252.
Stigmasterol has significant anti-arthritis and anti-inflammatory effects, but its role in immune and inflammatory diseases is still unclear.
The potential advantages of stigmasterol in asthma were explored in IL-13-induced BEAS-2B cells and asthmatic mice.
The optimal target of stigmasterol was confirmed in asthma. After detecting the cytotoxicity of stigmasterol in BEAS-2B cells, 10 μg/mL and 20 μg/mL stigmasterol were incubated with the BEAS-2B cell model for 48 h, and anti-inflammation and antioxidative stress were verified. Asthmatic mice were induced by OVA and received 100 mg/kg stigmasterol for 7 consecutive days. After 28 days, lung tissues and BAL fluid were collected for the following study. To further verify the role of NK1-R, 0.1 μM WIN62577 (NK1-R specific antagonist), and 1 μM recombinant human NK1-R protein were applied.
NK1-R was the potential target of stigmasterol. When the concentration of stigmasterol is 20 μg/mL, the survival rate of BEAS-2B cells is about 98.4%, which is non-toxic. Stigmasterol exerted anti-inflammation and antioxidant stress in a dose-dependent manner and decreased NK1-R expression in IL-13-induced BEAS-2B. Meanwhile, assay also indicated the anti-inflammation and antioxidant stress of stigmasterol after OVA challenge. Stigmasterol inhibited inflammation infiltration and mucus hypersecretion, and NK1-R expression.
The protective effect of stigmaterol on asthma and its underlying mechanism have been discussed in depth, providing a theoretical basis and more possibilities for its treatment of asthma.
豆甾醇具有显著的抗关节炎和抗炎作用，但它在免疫和炎症性疾病中的作用尚不清楚。
探讨豆甾醇在白介素-13诱导的 BEAS-2B 细胞和哮喘小鼠中的潜在优势。
确定豆甾醇在哮喘中的最佳靶点。在 BEAS-2B 细胞模型中检测豆甾醇的细胞毒性后，用 10μg/mL 和 20μg/mL 豆甾醇孵育 BEAS-2B 细胞 48 小时，验证抗炎和抗氧化应激作用。卵清蛋白（OVA）诱导哮喘小鼠，连续 7 天给予 100mg/kg 豆甾醇。28 天后，收集肺组织和 BAL 液进行后续研究。为了进一步验证 NK1-R 的作用，应用了 0.1μM WIN62577（NK1-R 特异性拮抗剂）和 1μM 重组人 NK1-R 蛋白。
NK1-R 是豆甾醇的潜在靶点。当豆甾醇浓度为 20μg/mL 时，BEAS-2B 细胞的存活率约为 98.4%，无毒性。豆甾醇呈剂量依赖性发挥抗炎和抗氧化应激作用，并降低 IL-13 诱导的 BEAS-2B 中 NK1-R 的表达。同时，实验还表明 OVA 挑战后豆甾醇具有抗炎和抗氧化应激作用。豆甾醇抑制炎症浸润和粘液分泌过度，以及 NK1-R 的表达。
深入探讨了豆甾醇对哮喘的保护作用及其潜在机制，为其治疗哮喘提供了理论依据和更多可能性。
