Mirsalari Marzieh, Elhami Shahla
Department of Chemistry, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
Department of Chemistry, Ahvaz Branch, Islamic Azad University, Ahvaz, Iran.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Oct 15;240:118617. doi: 10.1016/j.saa.2020.118617. Epub 2020 Jun 19.
In this study, graphene oxide/gold nanoparticles/Triton X-100 nanocomposites (GO/AuNPs/TX-100) were synthesized using the sonochemical method and their ability in ultrasound-assisted colorimetric detection of insulin was investigated. The synthesized GO/AuNPs/TX-100 nanocomposites were characterized by UV-visible absorption spectroscopy and TEM analysis. The interaction between the nanocomposites and insulin was observed by both naked eye and optical absorption spectroscopy. The GO/AuNPs/TX-100 nanocomposites displayed apparent color changes (red to blue) and absorption spectra changes (decreasing of the band around 528 nm and appearance of a new red-shifted band at 640 nm) in presence of insulin. The interaction mechanism of the nanocomposites and insulin was discussed. It is based on the special structure of insulin, that insulin can be easily self-assemble into the GO/AuNP/TX-100 nanocomposites and can also play the role of a bridge between two different GO/AuNPs/TX-100 nanocomposites by peptide chains. The effective parameters for insulin detection were optimized. The colorimetric method was used for quantification of insulin in the range of 2-300 ng mL with a detection limit of 0.1 ng mL. Moreover, the relative standard deviation of the method was 3.1 and 2.7% (n = 10) at concentrations of 50 and 200 ng mL, respectively on the same day and 4.8% at a concentration (200.0 ng mL) on five consecutive days. The present method was utilized for insulin assay in human blood serums with satisfactory results.
在本研究中，采用声化学方法合成了氧化石墨烯/金纳米粒子/曲拉通X-100纳米复合材料（GO/AuNPs/TX-100），并研究了其在超声辅助比色法检测胰岛素中的能力。通过紫外可见吸收光谱和透射电镜分析对合成的GO/AuNPs/TX-100纳米复合材料进行了表征。通过肉眼和光学吸收光谱观察了纳米复合材料与胰岛素之间的相互作用。在胰岛素存在下，GO/AuNPs/TX-100纳米复合材料呈现出明显的颜色变化（红色变为蓝色）和吸收光谱变化（528nm左右的谱带降低，640nm处出现新的红移谱带）。讨论了纳米复合材料与胰岛素的相互作用机制。基于胰岛素的特殊结构，胰岛素能够容易地自组装到GO/AuNP/TX-100纳米复合材料中，并且还能通过肽链在两种不同的GO/AuNPs/TX-100纳米复合材料之间起到桥梁作用。优化了胰岛素检测的有效参数。比色法用于定量检测2-300ng/mL范围内的胰岛素，检测限为0.1ng/mL。此外，该方法在同一天50ng/mL和200ng/mL浓度下的相对标准偏差分别为3.1%和2.7%（n=10），在连续五天200.0ng/mL浓度下的相对标准偏差为4.8%。该方法用于人血清中胰岛素的测定，结果令人满意。
