State Key Lab for Conservation and Utilization of Subtropical Agric-Biological Resources & Guangxi Key Lab for Sugarcane Biology & Academy of Sugarcane and Sugar Industry, Guangxi University, Nanning 530004, China.
IFAS Indian River Research and Education Center, University of Florida, Fort Pierce, FL 34945, USA.
Int J Mol Sci. 2023 Jun 29;24(13):10832. doi: 10.3390/ijms241310832.
Nitrogen availability might play an essential role in plant diseases by enhancing fungal cell growth and influencing the expression of genes required for successful pathogenesis. Nitrogen availability could modulate secondary metabolic pathways as evidenced by the significant differential expression of several core genes involved in mycotoxin biosynthesis and genes encoding polyketide synthase/nonribosomal peptide synthetases, cytochrome P450 and carbohydrate-active enzymes in , grown on different nitrogen sources. A combined analysis was carried out on the transcript and metabolite profiles of regulatory metabolic processes and the virulence of grown on various nitrogen sources. The nitrogen regulation of the gibberellin gene cluster included the metabolic flux and multiple steps of gibberellin synthesis. UHPLC-MS/MS-based metabolome analysis revealed the coordination of these related transcripts and the accumulation of gibberellin metabolites. This integrated analysis allowed us to uncover additional information for a more comprehensive understanding of biological events relevant to fungal secondary metabolic regulation in response to nitrogen availability.
氮素供应可能通过促进真菌细胞生长和影响成功发病所需基因的表达，在植物病害中发挥重要作用。氮素供应可以调节次级代谢途径，这一点可以从几种参与真菌毒素生物合成的核心基因和编码聚酮合酶/非核糖体肽合酶、细胞色素 P450 和碳水化合物活性酶的基因的差异表达中得到证明，这些基因在不同氮源上生长时存在显著差异。对不同氮源上生长的调控代谢过程和毒力的转录本和代谢物谱进行了联合分析。赤霉素基因簇的氮调控包括代谢通量和赤霉素合成的多个步骤。基于 UHPLC-MS/MS 的代谢组学分析揭示了这些相关转录本的协调和赤霉素代谢物的积累。这种综合分析使我们能够揭示更多信息，从而更全面地了解与氮素供应响应真菌次级代谢调控相关的生物事件。
