Taghavifar Saeed, Afroughi Fatemeh, Saadati Keyvan Maryam
Urmia University of Medical Sciences, Urmia, Iran.
Islamic Azad University, Tehran Medical Branch, Tehran, Iran.
Int J Low Extrem Wounds. 2022 Jun;21(2):141-153. doi: 10.1177/1534734620933079. Epub 2020 Jun 28.
Accurately orchestrated course of events normally observed in healing are not followed in diabetic wounds, and bacterial colonization/infection further messes up the process. Novel therapeutic options for treatment of infections caused by multidrug-resistant are urgently needed. HAMLET (human α-lactalbumin made lethal to tumor cells) has been reported to be able to sensitize bacterial pathogens to traditional antimicrobial agents. The aim was to assess the wound healing activity of curcumin nanoparticles in diabetic wounds infected with methicillin-resistant (MRSA) sensitized with HAMLET. Fifty male rats were randomized into 5 groups of 10 animals each. In CONTROL group, 0.1-mL sterile saline 0.9% solution was added to the wounds with no infection. In MRSA group, the wounds were infected with MRSA and only treated with 0.1-mL sterile saline 0.9% solution. In MRSA/HAMLET group, infected wounds were treated with HAMLET (100 µg). In MRSA/CNP group, animals with infected wounds were treated with 0.1 mL topical application of 1 mg/mL curcumin nanoparticles. In MRSA/CNP/HAMLET group, animals with infected wounds were treated with topical application of 0.1 mL solution of curcumin nanoparticles (1 mg/mL) and HAMLET (100 µg). All test formulations were applied for 10 days, twice a day, starting from first treatment. Microbiological examination; planimetric, biochemical, histological, and quantitative morphometric studies; immunohistochemical staining for angiogenesis; determination of hydroxyproline levels; and reverse transcription polymerase chain reaction for caspase 3, Bcl-2, and p53 showed that there was significant difference between animals in MRSA/CNP/HAMLET group compared with other groups ( < .05). Curcumin nanoparticles improved diabetic wounds infected with MRSA sensitized with HAMLET and had the potential to offer more attention to this safer agent for topical use in infected diabetic wounds.
在愈合过程中通常观察到的精确编排的一系列事件在糖尿病伤口中并未出现，并且细菌定植/感染进一步扰乱了这一过程。迫切需要针对耐多药感染的新型治疗选择。据报道，HAMLET（对肿瘤细胞具有致死性的人α-乳白蛋白）能够使细菌病原体对传统抗菌剂敏感。目的是评估姜黄素纳米颗粒对感染耐甲氧西林金黄色葡萄球菌（MRSA）并经HAMLET致敏的糖尿病伤口的愈合活性。五十只雄性大鼠被随机分为5组，每组10只动物。在对照组中，向未感染的伤口添加0.1 mL无菌0.9%生理盐水溶液。在MRSA组中，伤口感染MRSA，仅用0.1 mL无菌0.9%生理盐水溶液治疗。在MRSA/HAMLET组中，感染的伤口用HAMLET（100μg）治疗。在MRSA/CNP组中，感染伤口的动物用0.1 mL 1 mg/mL姜黄素纳米颗粒局部应用治疗。在MRSA/CNP/HAMLET组中，感染伤口的动物用0.1 mL姜黄素纳米颗粒（1 mg/mL）和HAMLET（100μg）溶液局部应用治疗。所有测试制剂从首次治疗开始，每天应用两次，持续10天。微生物学检查；平面测量、生化、组织学和定量形态学研究；血管生成的免疫组织化学染色；羟脯氨酸水平的测定；以及caspase 3、Bcl-2和p53的逆转录聚合酶链反应表明，与其他组相比，MRSA/CNP/HAMLET组的动物之间存在显著差异（P<0.05）。姜黄素纳米颗粒改善了感染经HAMLET致敏的MRSA的糖尿病伤口，并且有可能更多地关注这种更安全的局部用于感染性糖尿病伤口的药物。
