Ashja Zadeh Mohammad Ali, Ebrahimi Mohsen, Salarian Amir Ahmad, Abtahi Seyyed Reza, Jahandideh Alireza
Faculty of Medicine, AJA University of medical sciences, Tehran, Iran.
Department of Toxicology and Pharmacology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran.
Bull Emerg Trauma. 2020 Jul;8(3):169-178. doi: 10.30476/BEAT.2020.82567.
To evaluate the wound healing activity of leaves extract on infected wounds in diabetic rats.
Fifty male diabetic rats were randomized into two sets of 25 animals each. Each group was sub divided into five groups of five animals, each for excisional and incisional wound models, respectively. Induction of diabetes was achieved using 60 mg/kg streptozotocin. In group I, 0.1 mL sterile saline 0.9% solution was added to the wounds with no infection. In group II, the wounds were infected with Methicillin-resistant staphylococcus aureus (MRSA) and only treated with 0.1 mL the sterile saline 0.9% solution. In group III, infected wounds were treated with application of base formulation ointment. In group IV, animals with infected wounds were treated with 0.1 mL topical application of 1 mg/mL methicillin and base formulation ointment. In group V, animals with infected wounds were treated with topical application of 0.1 mL solution of methicillin (1 mg/mL) and with 1g of powder extract of the plant material in ointment. The healing of the wound was assessed based on planimetry, hydroxyproline estimation, microbiological, biomechanical and biochemical studies.
Microbiological examination, planimetric, histological and quantitative morphometric studies and determination of hydroxyproline levels showed that there was significant difference between animals in group V compared to other groups (=0.001). Biomechanical indices in incisional groups showed there was significant difference between animals in group V compared to other groups (=0.001).
It was possible to conclude that the ointment of the extract of . leaves have significant wound-healing activity in diabetes.
评估叶片提取物对糖尿病大鼠感染伤口的愈合活性。
将50只雄性糖尿病大鼠随机分为两组，每组25只。每组再分为五组，每组五只动物，分别用于切除伤口模型和切开伤口模型。使用60mg/kg链脲佐菌素诱导糖尿病。在第一组中，向伤口添加0.1mL无菌0.9%生理盐水溶液，伤口无感染。在第二组中，伤口感染耐甲氧西林金黄色葡萄球菌（MRSA），仅用0.1mL无菌0.9%生理盐水溶液治疗。在第三组中，感染伤口用基础配方软膏治疗。在第四组中，感染伤口的动物用0.1mL 1mg/mL甲氧西林局部应用和基础配方软膏治疗。在第五组中，感染伤口的动物用0.1mL甲氧西林（1mg/mL）溶液局部应用，并在软膏中加入1g植物材料粉末提取物治疗。基于平面测量、羟脯氨酸估计、微生物学、生物力学和生化研究评估伤口愈合情况。
微生物学检查、平面测量、组织学和定量形态学研究以及羟脯氨酸水平测定表明，与其他组相比，第五组动物之间存在显著差异（P=0.001）。切开伤口组的生物力学指标表明，与其他组相比，第五组动物之间存在显著差异（P=0.001）。
可以得出结论，该植物叶片提取物软膏对糖尿病具有显著的伤口愈合活性。
