Hekmat Manouchehr, Ahmadi Houssein, Baniasadi Farazad, Ashtari Behnaz, Naserzadeh Parvaneh, Mirzaei Mansooreh, Omidi Hamidreza, Mostafavinia Atarodalsadat, Amini Abdollah, Hamblin Michael R, Chien Sufan, Bayat Mohammad
Department of Cardiac Surgery, Shahid Modarres Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Biology and Anatomical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Lasers Med Sci. 2023 Jun 12;14:e18. doi: 10.34172/jlms.2023.18. eCollection 2023.
Here, we assess the therapeutic effects of photobiomodulation (PBM) and curcumin (CUR)-loaded superparamagnetic iron oxide nanoparticles (SPIONs), alone or together, on the maturation step of a type 1 diabetes (DM1) rat wound model. Full-thickness wounds were inflicted in 36 rats with diabetes mellitus (DM) induced by the administration of streptozotocin (STZ). The rats were randomly allocated to four groups. Group one was untreated (control); group two received CUR; group 3 received PBM (890 nm, 80 Hz, 0.2 J/cm); group 4 received a combination of PBM plus CUR. On days 0, 4, 7, and 15, we measured microbial flora, wound closure fraction, tensile strength, and stereological analysis. All treatment groups showed a substantial escalation in the wound closure rate, a substantial reduction in the count of methicillin-resistant (MRSA), a substantial improvement in wound strength, a substantially improvement in stereological parameters compared to the control group, however, the PBM+CUR group was superior to the other treatment groups (all, ≤0.05). All treatment groups showed significantly improved wound healing in the DM1 rat model. However, the PBM+CUR group was superior to the other treatment groups and the control group in terms of wound strength and stereological parameters.
在此，我们评估光生物调节（PBM）和负载姜黄素（CUR）的超顺磁性氧化铁纳米颗粒（SPIONs）单独或联合使用对1型糖尿病（DM1）大鼠伤口模型愈合阶段的治疗效果。通过注射链脲佐菌素（STZ）诱导36只大鼠患糖尿病，造成全层伤口。将大鼠随机分为四组。第一组不进行治疗（对照组）；第二组接受CUR治疗；第三组接受PBM治疗（890 nm，80 Hz，0.2 J/cm）；第四组接受PBM加CUR的联合治疗。在第0、4、7和15天，我们测量了微生物菌群、伤口闭合率、抗张强度和体视学分析。与对照组相比，所有治疗组的伤口闭合率均显著提高，耐甲氧西林金黄色葡萄球菌（MRSA）数量显著减少，伤口强度显著改善，体视学参数也有显著改善，然而，PBM+CUR组优于其他治疗组（所有P值均≤0.05）。所有治疗组在DM1大鼠模型中均显示出伤口愈合显著改善。然而，在伤口强度和体视学参数方面，PBM+CUR组优于其他治疗组和对照组。
