Hsu Po-Chih, Cheng Ching-Feng, Hsieh Po-Chun, Chen Yi-Hsuan, Kuo Chan-Yen, Sytwu Huey-Kang
Graduate Institute of Medical Sciences, National Defense Medical Center, Taipei, Taiwan.
Department of Dentistry, Taipei Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, New Taipei City, Taiwan.
Evid Based Complement Alternat Med. 2020 May 26;2020:5867064. doi: 10.1155/2020/5867064. eCollection 2020.
Oral cancer belongs to the class of head and neck cancers and can be life threatening if not diagnosed and treated early. Activation of cell death via apoptosis or reactive oxygen species (ROS) accumulation and inhibition of cell cycle progression, migration, and epithelial-to-mesenchymal transition (EMT) may be a good strategy to arrest the development of oral cancer. In this study, we analyzed the possible action of chrysophanol isolated from the rhizomes of on the oral cancer cell lines FaDu (human pharynx squamous cell carcinoma) and SAS (human tongue squamous carcinoma) by investigating whether chrysophanol could influence cell death.
Cell viability was measured by using the MTT assay. For the detection of apoptosis, terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining and subG1 population analysis were used. We also examined cell cycle progression and ROS levels by flow cytometry. Additionally, the expression of p53, p21, procaspase 3, cyclin D1, CDK4, cdc2, CDK2, E-cadherin, vimentin, and PCNA was evaluated by western blotting.
Chrysophanol has an anticancer effect on FaDu and SAS cell lines. There is an increase in subG1 accumulation, ROS production, and cell cycle G1 arrest after treatment with chrysophanol. On the other hand, chrysophanol inhibited cell migration/metastasis and EMT. We proposed that chrysophanol may be a good candidate compound on oral cancer treatment in the further.
口腔癌属于头颈癌范畴，若不及早诊断和治疗可能危及生命。通过凋亡或活性氧（ROS）积累激活细胞死亡以及抑制细胞周期进程、迁移和上皮-间质转化（EMT）可能是阻止口腔癌发展的良好策略。在本研究中，我们通过研究大黄酚是否能影响细胞死亡，分析了从大黄根茎中分离出的大黄酚对口腔癌细胞系FaDu（人咽鳞状细胞癌）和SAS（人舌鳞状癌）的可能作用。
使用MTT法测定细胞活力。采用末端脱氧核苷酸转移酶dUTP缺口末端标记（TUNEL）染色和亚G1期细胞群分析检测细胞凋亡。我们还通过流式细胞术检测细胞周期进程和ROS水平。此外，通过蛋白质免疫印迹法评估p53、p21、procaspase 3、细胞周期蛋白D1、细胞周期蛋白依赖性激酶4（CDK4）、细胞周期蛋白依赖性激酶2（cdc2）、细胞周期蛋白依赖性激酶2（CDK2）、E-钙黏蛋白、波形蛋白和增殖细胞核抗原（PCNA）的表达。
大黄酚对FaDu和SAS细胞系具有抗癌作用。用大黄酚处理后，亚G1期细胞积累、ROS产生增加以及细胞周期G1期阻滞。另一方面，大黄酚抑制细胞迁移/转移和EMT。我们提出大黄酚在未来可能是口腔癌治疗的良好候选化合物。
