Zhang Jing, Li Yongzheng, Li Jialu, Shi Yuan, Hu Jinxing, Yang Guoli
The Affiliated Stomatology Hospital, Zhejiang University School of Medicine, Hangzhou, China.
Key Laboratory of Oral Biomedical Research of Zhejiang Province, Zhejiang University School of Stomatology, Hangzhou, China.
Front Bioeng Biotechnol. 2020 Jun 11;8:624. doi: 10.3389/fbioe.2020.00624. eCollection 2020.
The peri-implant epithelium (PIE) forms a crucial seal between the oral environment and the implant surface. Compared with the junctional epithelium (JE), the biological sealing of PIE is fragile, which lacks hemidesmosomes (HDs) and internal basal lamina (extracellular matrix containing laminin332, IBL) on the upper part of the interface. In the study, we aim to prepare a coating with good biocompatibility and ability to immobilize the recombinant adenovirus vector of LAMA3 (AdLAMA3) for promoting the re-epithelization of PIE. The titanium surface functionalized with AdLAMA3 was established via layer-by-layer assembly technique and antibody-antigen specific binding. The biological evaluations including cell adhesion and the re-epithelization of PIE were investigated. The results demonstrated that the AdLAMA3 coating could improve epithelial cell attachment and cell spreading in the early stage. experiments indicated that the AdLAMA3 coating on the implant surface has the potential to accelerate the healing of the PIE, and could promote the expression of laminin α3 and the formation of hemidesmosomes. This study might provide a novel approach and experimental evidence for the precise attachment of LAMA3 to titanium surfaces. The process could improve the re-epithelization of PIE.
种植体周围上皮（PIE）在口腔环境与种植体表面之间形成关键的密封。与结合上皮（JE）相比，PIE的生物密封较为脆弱，其界面上部缺乏半桥粒（HDs）和内部基膜（含层粘连蛋白332的细胞外基质，IBL）。在本研究中，我们旨在制备一种具有良好生物相容性且能够固定LAMA3重组腺病毒载体（AdLAMA3）的涂层，以促进PIE的再上皮化。通过层层组装技术和抗体-抗原特异性结合建立了用AdLAMA3功能化的钛表面。研究了包括细胞黏附及PIE再上皮化在内的生物学评价。结果表明，AdLAMA3涂层可在早期改善上皮细胞附着和细胞铺展。实验表明，种植体表面的AdLAMA3涂层有加速PIE愈合的潜力，并可促进层粘连蛋白α3的表达和半桥粒的形成。本研究可能为LAMA3精确附着于钛表面提供一种新方法和实验证据。该过程可改善PIE的再上皮化。
