Pham Maria H, Haugen Håvard J, Rinna Alessandra, Ellingsen Jan Eirik, Reseland Janne E
Department of Biomaterials, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
Department of Prosthodontics, Institute of Clinical Dentistry, University of Oslo, Oslo, Norway.
J Tissue Eng. 2019 Feb 8;10:2041731419828950. doi: 10.1177/2041731419828950. eCollection 2019 Jan-Dec.
The attachment of implants relies on bone and soft tissue biocompatibility. The aim of this article is to investigate the effect of fluoride-modified metallic titanium (Ti) surfaces (Ti-F) on proliferation and differentiation of human gingival fibroblasts. Human gingival fibroblast cells were exposed to hydrofluoric acid-modified Ti coins (Ti-F) for 1, 3, 7, 14 and 21 days, and untreated coins were used as controls. A five- to six-fold increase in the proliferation of human gingival fibroblasts on Ti-F compared to Ti surfaces was observed. Enhanced gene expression of interleukin-6 and osteoprotegerin was found at 7 days. Increased levels of sclerostin, interleukin-6 and osteoprotegerin in the media from human gingival fibroblasts cultured on Ti-F coins were found compared to controls. Our results confirm that hydrofluoric acid-modified surface may indirectly enhance the firm attachment of implant surface to junction epithelium, soft tissue epithelium, which would give protection for underlying osseous structures making osseointegration of the dental implant possible.
植入物的附着依赖于骨和软组织的生物相容性。本文旨在研究氟改性金属钛（Ti）表面（Ti-F）对人牙龈成纤维细胞增殖和分化的影响。将人牙龈成纤维细胞暴露于氢氟酸改性的Ti硬币（Ti-F）1、3、7、14和21天，未处理的硬币用作对照。与Ti表面相比，观察到Ti-F上的人牙龈成纤维细胞增殖增加了五到六倍。在第7天发现白细胞介素-6和骨保护素的基因表达增强。与对照相比，在Ti-F硬币上培养的人牙龈成纤维细胞培养基中硬化蛋白、白细胞介素-6和骨保护素的水平升高。我们的结果证实，氢氟酸改性表面可能间接增强植入物表面与结合上皮、软组织上皮的牢固附着，这将为下方的骨结构提供保护，使牙种植体的骨整合成为可能。
