Department of Biochemistry & Biophysics and Center for RNA Biology, School of Medicine and Dentistry, University of Rochester, Rochester, New York, USA.
Wiley Interdiscip Rev RNA. 2021 Mar;12(2):e1611. doi: 10.1002/wrna.1611. Epub 2020 Jun 29.
The 5' cap and 3' poly(A) tail of mRNA are known to synergistically regulate mRNA translation and stability. Recent computational and experimental studies revealed that both protein-coding and non-coding RNAs will fold with extensive intramolecular secondary structure, which will result in close distances between the sequence ends. This proximity of the ends is a sequence-independent, universal property of most RNAs. Only low-complexity sequences without guanosines are without secondary structure and exhibit end-to-end distances expected for RNA random coils. The innate proximity of RNA ends might have important biological implications that remain unexplored. In particular, the inherent compactness of mRNA might regulate translation initiation by facilitating the formation of protein complexes that bridge mRNA 5' and 3' ends. Additionally, the proximity of mRNA ends might mediate coupling of 3' deadenylation to 5' end mRNA decay. This article is categorized under: RNA Structure and Dynamics > RNA Structure, Dynamics, and Chemistry RNA Structure and Dynamics > Influence of RNA Structure in Biological Systems Translation > Translation Regulation.
mRNA 的 5' 帽和 3' 聚（A）尾协同调节 mRNA 的翻译和稳定性。最近的计算和实验研究表明，蛋白质编码和非编码 RNA 都会折叠成广泛的分子内二级结构，从而导致序列末端之间的距离非常接近。这种末端的接近是大多数 RNA 的一种非序列依赖的、普遍的特性。只有没有鸟嘌呤的低复杂度序列没有二级结构，表现出 RNA 无规卷曲的末端到末端的预期距离。RNA 末端的固有接近性可能具有尚未探索的重要生物学意义。特别是，mRNA 的固有紧凑性可能通过促进桥接 mRNA 5' 和 3' 末端的蛋白质复合物的形成来调节翻译起始。此外，mRNA 末端的接近可能介导 3' 脱腺苷酸化与 5' 端 mRNA 降解的偶联。本文属于以下类别：RNA 结构和动态 > RNA 结构、动态和化学 RNA 结构和动态 > RNA 结构对生物系统的影响 翻译 > 翻译调控。
