School of Chemistry, State Key Laboratory of Electrical Insulation and Power Equipments, MOE Key Laboratory for Non-Equilibrium Synthesis and Modulation of Condensed Matter, Xi'an Jiaotong University, Xi'an, Shaanxi, P. R. China.
Institute of Medical Engineering, Department of Biophysics, School of Basic Medical Sciences, Xi'an Jiaotong University Health Science Center, Xi'an, Shaanxi, P. R. China.
Soft Matter. 2020 Jul 22;16(28):6591-6598. doi: 10.1039/d0sm00593b.
The fabrication of scaffolds with suitable chemical, physical, and electrical properties is critical for nerve cell adhesion and proliferation. Recently, electrical stimulation on conductive polymers has been applied to construct functional nerve cell scaffolds. Herein, we prepared natural polymer (cellulose)/conductive polymer nanofibrous mats, i.e., electrospun cellulose (EC)/poly N-vinylpyrrole (PNVPY) and EC/poly(3-hexylthiophene) (P3HT) through an efficient in situ polymerization method. The surface immobilization was characterized by optical microscopy (OM), scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, hydrophilicity, porosity, and cyclic voltammetry. The OM and SEM images showed that PNVPY formed polymer coatings and aggregated nanoparticles on the EC nanofibers, while P3HT only produced polymer coatings. Compared with pure EC mats, both the composite mats had increased thickness, higher porosity, and higher conductivity. Also, an increase in hydrophilicity was found for EC/P3HT. In vivo cytocompatibility of the undifferentiated PC12 cells showed that the EC/PNVPY and EC/P3HT scaffolds exhibited favorable cell activity, adhesion, and proliferation. Furthermore, the results of electrical stimulation experiments indicated that the EC/P3HT mats could effectively promote the proliferation of the PC12 cells more than the EC and EC/PNVPY mats. The findings suggest a positive outcome regarding the conductive polymer-modified EC/PNVPY and EC/P3HT nanofibrous mats in neural tissue engineering.
制备具有合适化学、物理和电学性能的支架对于神经细胞的黏附和增殖至关重要。最近，对导电聚合物施加电刺激已被应用于构建功能性神经细胞支架。在此，我们通过一种有效的原位聚合方法制备了天然聚合物（纤维素）/导电聚合物纳米纤维垫，即静电纺丝纤维素（EC）/聚 N-乙烯基吡咯烷酮（PNVPY）和 EC/聚（3-己基噻吩）（P3HT）。表面固定化通过光学显微镜（OM）、扫描电子显微镜（SEM）、傅里叶变换红外（FTIR）光谱、亲水性、孔隙率和循环伏安法进行了表征。OM 和 SEM 图像表明，PNVPY 在 EC 纳米纤维上形成了聚合物涂层和聚集的纳米颗粒，而 P3HT 仅产生了聚合物涂层。与纯 EC 垫相比，复合垫的厚度增加，孔隙率和导电性更高。同时，还发现 EC/P3HT 的亲水性增加。未分化的 PC12 细胞的体内细胞相容性表明，EC/PNVPY 和 EC/P3HT 支架表现出良好的细胞活性、黏附和增殖。此外，电刺激实验的结果表明，EC/P3HT 垫比 EC 和 EC/PNVPY 垫更能有效促进 PC12 细胞的增殖。这些发现表明，导电聚合物修饰的 EC/PNVPY 和 EC/P3HT 纳米纤维垫在神经组织工程中具有积极的效果。
