Seymenska Daniela, Teneva Desislava, Nikolova Irina, Benbassat Niko, Denev Petko
Department of Pharmacognosy and Pharmaceutical Chemistry, Faculty of Pharmacy, Medical University-Plovdiv, 4002 Plovdiv, Bulgaria.
Laboratory of Biologically Active Substances, Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, 139 Ruski Blvd., 4000 Plovdiv, Bulgaria.
Pharmaceuticals (Basel). 2024 Mar 23;17(4):409. doi: 10.3390/ph17040409.
L. (, SN) or black elder is a traditional medicinal plant widely used worldwide for therapeutic and dietary purposes. The aim of the current study was to investigate the anti-inflammatory and antinociceptive activities of black elder fruit and flower extracts (SNFrE and SNFlE, respectively). The primary polyphenol constituents in the flower extract were flavonoids and phenolic acids, while anthocyanins were the main components in the fruit extract. SNFrE revealed pronounced and dose-dependent in vivo anti-inflammatory activity assessed by the cotton pellet-induced granuloma test. Doses of 10, 20, and 50 mg/kg BW of SNFrE reduced the weight of induced granuloma in rats by 20.3%, 20.5%, and 28.4%, respectively. At the highest dose (50 mg/kg BW), SNFrE had significant ( < 0.01) anti-inflammatory activity comparable to that of diclofenac, the reference compound used (10 mg/kg BW). In addition, the in vivo antinociceptive activity of the extracts in mice was estimated using the acetic-acid-induced writhing test. Both extracts at doses of 50 mg/kg BW inhibited the abdominal contractions induced by the acetic acid significantly comparing to the control group ( < 0.01). Our findings indicate that black elder extracts and particularly SNFrE possess anti-inflammatory and antinociceptive activities, providing experimental evidence for the use of in traditional medicine.
接骨木（L. nigra，SN）或黑接骨木是一种传统药用植物，在全球范围内广泛用于治疗和饮食目的。本研究的目的是研究黑接骨木果实和花朵提取物（分别为SNFrE和SNFlE）的抗炎和镇痛活性。花朵提取物中的主要多酚成分是黄酮类化合物和酚酸，而花色苷是果实提取物中的主要成分。通过棉球诱导的肉芽肿试验评估，SNFrE显示出明显的剂量依赖性体内抗炎活性。SNFrE剂量为10、20和50mg/kg体重时，大鼠诱导肉芽肿的重量分别降低了20.3%、20.5%和28.4%。在最高剂量（50mg/kg体重）下，SNFrE具有显著（P<0.01）的抗炎活性，与所用参考化合物双氯芬酸（10mg/kg体重）相当。此外，使用乙酸诱导的扭体试验评估提取物在小鼠体内的镇痛活性。与对照组相比，两种提取物剂量为50mg/kg体重时均显著抑制了乙酸诱导的腹部收缩（P<0.01）。我们的研究结果表明，黑接骨木提取物，尤其是SNFrE具有抗炎和镇痛活性，为其在传统医学中的应用提供了实验证据。
