School of Chemistry and Chemical Engineering, Jiangsu University, Zhenjiang, 212013, China.
School of Chemistry and Chemical Engineering, Yancheng Institute of Technology, Yancheng, 224051, China.
Mikrochim Acta. 2020 Jun 29;187(7):410. doi: 10.1007/s00604-020-04350-3.
Bimetallic Ag-Pt nanoparticles decorated on the surface of reduced graphene oxide (Ag-Pt/rGO) were designed and selected as a nanozyme for the assay of hydrogen peroxide. The nanocomposites were prepared through a one-pot reduction of potassium chloroplatinate, silver nitrate, and graphene oxide under ultraviolet irradiation without using any extra chemical reducing agents or surfactants. The successful formation of Ag-Pt/rGO nanocomposites was confirmed by transmission electron microscopy, energy disperse spectroscopy mapping, X-ray photoelectron spectroscopy, and X-ray diffraction analysis. Significantly, Ag-Pt/rGO nanocomposites possessed excellent peroxidase-like activity toward the catalytic oxidation of 3,3',5,5'-tetramethylbenzidine to form a blue product in the presence of hydrogen peroxide. Steady-state kinetics studies suggested that Ag-Pt/rGO nanocomposites had high affinity to hydrogen peroxide. Based on these properties, a convenient and sensitive method for the colorimetric determination of hydrogen peroxide was developed. Under optimal conditions, the absorbance at 652 nm increases linearly in the 10-100 μM and 100 μM-1 mM ranges of hydrogen peroxide concentration, and the detection limit is 0.9 μM (S/N = 3). The method was successfully applied to the determination of hydrogen peroxide in real water samples. Graphical abstract Ag-Pt/rGO nanocomposites were prepared by a one-pot UV irradiation method and used as a novel nanozyme for colorimetric determination of HO.
双金属 Ag-Pt 纳米粒子修饰在还原氧化石墨烯（Ag-Pt/rGO）的表面上被设计并选择作为纳米酶用于测定过氧化氢。该纳米复合材料通过在没有使用任何额外的化学还原剂或表面活性剂的情况下，在紫外光照射下一锅还原氯铂酸钾、硝酸银和氧化石墨烯制备而成。通过透射电子显微镜、能量色散光谱映射、X 射线光电子能谱和 X 射线衍射分析证实了 Ag-Pt/rGO 纳米复合材料的成功形成。显著的是，Ag-Pt/rGO 纳米复合材料在存在过氧化氢的情况下对 3,3',5,5'-四甲基联苯胺的催化氧化具有优异的过氧化物酶样活性，形成蓝色产物。稳态动力学研究表明，Ag-Pt/rGO 纳米复合材料对过氧化氢具有高亲和力。基于这些性质，开发了一种用于过氧化氢比色测定的简便灵敏的方法。在最佳条件下，在 10-100 μM 和 100 μM-1 mM 过氧化氢浓度范围内，652nm 处的吸光度呈线性增加，检测限为 0.9 μM（S/N=3）。该方法成功应用于实际水样中过氧化氢的测定。
