College of Chemistry, Guangdong University of Petrochemical Technology, Guandu Road, Maoming, Guangdong, 525000, People's Republic of China.
Guangxi Key Laboratory of Information Materials, School of Life and Environmental Sciences, Guilin University of Electronic Technology, Guilin, Guangxi, 541004, People's Republic of China.
Anal Bioanal Chem. 2023 Dec;415(29-30):7103-7115. doi: 10.1007/s00216-023-04975-2. Epub 2023 Oct 14.
1,5-anhydroglucitol (1,5-AG) is of considerable clinical relevance as a biochemical marker of glucose metabolism in the assessment and monitoring of diabetes. Herein, a simple colorimetric biosensor was constructed for the identification and detection of 1,5-AG by using pyranose oxidase (PROD) enzyme cascaded with reduced graphene oxide/persimmon tannin/Pt@Pd (RGO-PT/Pt@Pd NPs) nanozyme. The as-prepared RGO-PT/Pt@Pd NPs had excellent peroxidase-like activity and can be applied as a nanozyme. First, PROD enzyme reacts with the target 1,5-AG, decomposing 1,5-AG into 1,5-anhydrofuctose (1,5-AF) and HO. At this point, the highly catalytic RGO-PT/Pt@Pd NPs nanozyme produces a cascade with PROD enzyme which catalyzes the decomposition of HO to produce O. This in turn oxidizes the substrate 3,3',5,5'-tetramethylbenzidine (TMB) and produces a color change in the solution. Finally, the detection of 1,5-AG was achieved by measuring the absorption peak at 652 nm with an ultraviolet visible (UV-vis) spectrophotometer. Under optimal conditions, the linear operating range of the 1,5-AG enzyme cascade colorimetric sensor was 1.0-100.0 μg/mL, and the limit of detection (LOD) was 0.81 μg/mL. The proposed colorimetric biosensor was successfully applied to detect 1,5-AG in spiked human serum samples with the recoveries of 97.2-103.9% and RSDs of 1.94-4.48%. It provides a promising developmental assay for clinical detection of 1,5-AG.
1,5-脱水葡萄糖醇（1,5-AG）作为葡萄糖代谢的生化标志物，在糖尿病的评估和监测中具有重要的临床意义。本文构建了一种简单的比色生物传感器，用于通过使用吡喃糖氧化酶（PROD）酶级联还原氧化石墨烯/柿子单宁/Pt@Pd（RGO-PT/Pt@Pd NPs）纳米酶来识别和检测 1,5-AG。所制备的 RGO-PT/Pt@Pd NPs 具有优异的过氧化物酶样活性，可作为纳米酶应用。首先，PROD 酶与目标物 1,5-AG 反应，将 1,5-AG 分解为 1,5-脱水果糖（1,5-AF）和 HO。此时，高催化活性的 RGO-PT/Pt@Pd NPs 纳米酶与 PROD 酶发生级联反应，PROD 酶催化 HO 分解生成 O。这反过来又氧化底物 3,3',5,5'-四甲基联苯胺（TMB），使溶液发生颜色变化。最后，通过用紫外可见（UV-vis）分光光度计测量 652nm 处的吸收峰来实现 1,5-AG 的检测。在最佳条件下，1,5-AG 酶级联比色传感器的线性工作范围为 1.0-100.0μg/mL，检测限（LOD）为 0.81μg/mL。该比色生物传感器成功应用于检测加标人血清样品中的 1,5-AG，回收率为 97.2-103.9%，相对标准偏差（RSD）为 1.94-4.48%。它为临床检测 1,5-AG 提供了一种有前途的发展分析方法。
