Nano-Bio Regenerative Medical Institute, College of Medicine, Hallym University, Chuncheon, Republic of Korea.
Departments of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, School of Medicine, Hallym University, Chuncheon, Republic of Korea.
Adv Exp Med Biol. 2020;1249:53-66. doi: 10.1007/978-981-15-3258-0_4.
Three-dimensional (3D) bioprinting has been a highly influential technology in the field of tissue engineering to enable speedy and precise spatial patterning of cells, growth factors, and biomaterials. Bioink is one of the main factors in 3D bioprinting, and hydrogels are excellent matrix type by means of bioinks for 3D bioprinting. Recently, stereolithographic bioprinting via digital light processing (DLP) that allows high spatial resolution and rapid printing time of complex structures has attracted many studies. However, a small number of bioinks have been applied to DLP bioprinting in comparison with bioinks for other bioprinters. We developed a novel bioink based on silk fibroin that has been extensively used in biomedical fields due to its positive biological and biochemical properties as biomaterials. In this chapter, we summarized the silk fibroin basics and various applications of silk fibroin as printing material. Also, fabrication and performance of silk-based bioink for DLP bioprinter were discussed.
三维（3D）生物打印技术在组织工程领域具有重要的影响力，它可以实现细胞、生长因子和生物材料的快速、精确的空间图案化。生物墨水是 3D 生物打印的主要因素之一，而水凝胶则是生物墨水用于 3D 生物打印的优秀基质类型。最近，通过数字光处理（DLP）的立体光刻生物打印技术因其能够实现复杂结构的高空间分辨率和快速打印时间而吸引了许多研究。然而，与其他生物打印机的生物墨水相比，应用于 DLP 生物打印的生物墨水数量较少。我们开发了一种基于丝素蛋白的新型生物墨水，由于其作为生物材料的积极的生物和生化特性，已广泛应用于生物医学领域。在本章中，我们总结了丝素蛋白的基础知识以及丝素蛋白作为打印材料的各种应用。此外，还讨论了用于 DLP 生物打印机的丝基生物墨水的制造和性能。
