Department of Organic Materials Engineering, Chungnam National University, Daejeon 34134, South Korea.
Department of Nature-Inspired Nanoconvergence Systems, Korea Institute of Machinery and Materials, Daejeon 34103, South Korea.
Biomacromolecules. 2021 May 10;22(5):1921-1931. doi: 10.1021/acs.biomac.1c00034. Epub 2021 Apr 11.
Three-dimensional (3D) bioprinting is a technology under active study for use in tissue engineering and regenerative medicine. Bioink comprises cells and polymers and is the essential material for 3D bioprinting. The characteristics of the bioink affect its printability, gelation behavior, and cell compatibility. In this study, alginate derivatives were synthesized to induce rapid gelation, and a bioink was prepared by mixing these alginate derivatives with silk fibroin to enhance cell compatibility. A low-concentration (3 wt %) alginate/silk fibroin (Alg/SF) bioink was pregelated by the ionic cross-linking of Alg to increase the viscosity for 3D printing. The rheological and mechanical properties were analyzed using a rheometer and a texture meter, respectively. Analysis of cell viability and proliferation using fibroblasts (NIH-3T3) in the bioinks showed that the Alg/SF bioink has improved cytocompatibility compared to that of conventional Alg bioinks, making it a promising material for tissue engineering.
三维（3D）生物打印技术是组织工程和再生医学领域的一项热门研究技术。生物墨水由细胞和聚合物组成，是 3D 生物打印的关键材料。生物墨水的特性会影响其可打印性、凝胶化行为和细胞相容性。在本研究中，我们合成了海藻酸盐衍生物以诱导快速凝胶化，并将这些海藻酸盐衍生物与丝素蛋白混合制备生物墨水，以提高细胞相容性。通过 Alg 的离子交联将低浓度（3wt%）的藻酸盐/丝素蛋白（Alg/SF）生物墨水预凝胶化，以增加其用于 3D 打印的粘度。使用流变仪和质构仪分别分析了流变和力学性能。通过在生物墨水中使用成纤维细胞（NIH-3T3）分析细胞活力和增殖情况表明，与传统的 Alg 生物墨水相比，Alg/SF 生物墨水具有更好的细胞相容性，有望成为组织工程的理想材料。
