Institute for Cancer Genetics, Columbia University, New York, NY.
Department of Pathology and Cell Biology, Columbia University, New York, NY.
J Exp Med. 2020 Oct 5;217(10). doi: 10.1084/jem.20200483.
In response to T cell-dependent antigens, mature B cells are stimulated to form germinal centers (GCs), the sites of B cell affinity maturation and the cell of origin (COO) of most B cell lymphomas. To explore the dynamics of GC B cell development beyond the known dark zone and light zone compartments, we performed single-cell (sc) transcriptomic analysis on human GC B cells and identified multiple functionally linked subpopulations, including the distinct precursors of memory B cells and plasma cells. The gene expression signatures associated with these GC subpopulations were effective in providing a sc-COO for ∼80% of diffuse large B cell lymphomas (DLBCLs) and identified novel prognostic subgroups of DLBCL.
针对 T 细胞依赖性抗原，成熟 B 细胞受到刺激形成生发中心（GC），这是 B 细胞亲和力成熟的部位，也是大多数 B 细胞淋巴瘤的细胞起源（COO）。为了探索 GC B 细胞发育的动态超出已知的暗区和亮区区室，我们对人类 GC B 细胞进行了单细胞（sc）转录组分析，确定了多个功能相关的亚群，包括记忆 B 细胞和浆细胞的不同前体。与这些 GC 亚群相关的基因表达特征可有效地为大约 80%的弥漫性大 B 细胞淋巴瘤（DLBCL）提供 sc-COO，并确定了 DLBCL 的新的预后亚组。
