Department of Natural Sciences, Faculty of Science and Technology, Middlesex University, The Burroughs, Hendon, London NW4 4BT, UK; Molecular Parasitology Laboratory, School of Life Sciences, Kingston University, Kingston upon Thames, Surrey KT1 2EE, UK; The Department of Life Science, Natural History Museum London, Cromwell Road, London, SW7 5BD, UK.
Department of Natural Sciences, Faculty of Science and Technology, Middlesex University, The Burroughs, Hendon, London NW4 4BT, UK.
Parasitol Int. 2020 Dec;79:102166. doi: 10.1016/j.parint.2020.102166. Epub 2020 Jun 27.
Despite Diplostomum baeri (Dubois, 1937) being one of the most widely distributed parasites of freshwater fish, there is no complete mitochondrial (mt) genome currently available. The complicated systematics presented by D. baeri has hampered investigations into the species distributions and infective dynamics of the species. Within this study we obtained complete mt genome sequences of D. baeri and assessed its phylogenetic relationship with other species of Digenea. The complete mitochondrial genome of D. baeri is 14,480 bp in length, containing 36 genes in total. The phylogenetic tree resulting from Bayesian inference of concatenated 12 protein coding gene sequences placed D. baeri alongside published mt genomes of Diplostomidae, with the overall taxonomic placement of the genus being a sister lineage of the order Plagiochiida The characterization of further mitochondrial genomes within the family Diplostomidae will help progress phylogenetic and epidemiological investigations as well as providing a framework for the analysis of diagnostic markers to be used in further monitoring of the parasite worldwide.
尽管双腔吸虫（Dubois，1937）是淡水鱼类中分布最广的寄生虫之一，但目前尚无完整的线粒体（mt）基因组。D. baeri 复杂的系统发育阻碍了对该物种分布和感染动态的研究。在本研究中，我们获得了 D. baeri 的完整 mt 基因组序列，并评估了其与其他 Digenea 物种的系统发育关系。D. baeri 的完整线粒体基因组长 14480bp，共包含 36 个基因。基于 12 个蛋白编码基因序列的联合贝叶斯推断得到的系统发育树将 D. baeri 与已发表的双腔科 mt 基因组放在一起，属的总体分类位置是 Plagiochiida 目的姐妹谱系。进一步研究双腔科内的线粒体基因组将有助于推进系统发育和流行病学研究，并为分析用于进一步监测全球寄生虫的诊断标记物提供框架。
