Jimenez Chavez C Leonardo, Coelho Michal A, Brewin Lindsey W, Swauncy Isaiah, Tran Tori, Albanese Taylor, Laguna Angie, Gabriela Ivette, Szumlinski Karen K
Department of Psychological and Brain Sciences, University of California Santa Barbara, Santa Barbara, CA 93196-9660, USA.
Department of Psychology, California State University Dominguez Hills, Carson, CA 90747-0001, USA.
Brain Sci. 2020 Jun 26;10(6):405. doi: 10.3390/brainsci10060405.
A prior history of excessive drinking induces a negative affective state in both humans and laboratory rodents, the manifestation of which varies with the age of drinking-onset. In adolescent male mice, negative affect incubates over the course of a 30-day alcohol withdrawal period. In contrast, the negative affect exhibited by adult male mice is robust at 1 day withdrawal, but dissipates with the passage of time. As females tend to consume more alcohol than males, we aimed to explore the affective disturbances exhibited by adolescent and adult C57BL/6J mice of both sexes during more protracted alcohol withdrawal and to relate any behavioral changes observed to plasma corticosterone levels as a biochemical index of stress. Male and female, adolescent and adult, mice were subjected to 14 consecutive days of binge alcohol-drinking using a multi-bottle-choice Drinking-in-the-Dark (DID) procedure (5, 10, 20 and 40% /). Age- and sex-matched control mice consumed water only. On either withdrawal day 1 or 70, subgroups of animals were subjected a to 1-day behavioral test battery that included the light-dark box shuttle test, marble-burying test, and Porsolt forced swim test. As expected, adolescent mice consumed more alcohol than adults and females consumed more alcohol than males. However, despite binge-like levels of alcohol consumption, we detected relatively few signs of alcohol withdrawal-induced negative affect and there was no correlation between affective behavior and circulating corticosterone levels. We discuss these findings within the context of our published work, highlighting procedural differences that might account for the relatively weak effect of binge-drinking history upon anxiety and depressive-like behavior observed herein.
既往过度饮酒史会在人类和实验啮齿动物中诱发负面情绪状态，其表现会因饮酒起始年龄的不同而有所变化。在青春期雄性小鼠中，负面情绪会在30天的酒精戒断期内逐渐显现。相比之下，成年雄性小鼠表现出的负面情绪在戒断1天时较为强烈，但会随着时间的推移而消散。由于雌性往往比雄性摄入更多的酒精，我们旨在探究青春期和成年期的C57BL/6J雌雄小鼠在更长时间的酒精戒断过程中所表现出的情感障碍，并将观察到的任何行为变化与作为应激生化指标的血浆皮质酮水平联系起来。使用多瓶选择黑暗中饮酒（DID）程序（5%、10%、20%和40%酒精），对雄性和雌性、青春期和成年期的小鼠进行连续14天的暴饮酒精实验。年龄和性别匹配的对照小鼠只饮水。在戒断第1天或第70天，将动物亚组进行为期1天的行为测试组合，包括明暗箱穿梭试验、埋大理石试验和波索尔特强迫游泳试验。正如预期的那样，青春期小鼠比成年小鼠摄入更多的酒精，雌性比雄性摄入更多的酒精。然而，尽管酒精摄入量达到暴饮水平，但我们检测到酒精戒断诱发的负面情绪迹象相对较少，且情感行为与循环皮质酮水平之间没有相关性。我们将在已发表的研究背景下讨论这些发现，强调可能导致本文观察到的暴饮饮酒史对焦虑和抑郁样行为影响相对较弱的程序差异。
