Lin Chao-Nan, Ke Ni-Jyun, Chiou Ming-Tang
Animal Disease Diagnostic Center, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Department of Veterinary Medicine, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, Taiwan.
Vaccines (Basel). 2020 Jun 26;8(2):339. doi: 10.3390/vaccines8020339.
Porcine circovirus-associated diseases (PCVADs) cause considerable economic losses in industrial pork production in the field. To minimize the economic losses due to PCVAD, porcine circovirus type 2 (PCV2) vaccines have been developed, and there is widespread vaccination worldwide today. However, limited information is available concerning the current status of PCV2 infection in the field on the Asian continent. The present study aimed to assess sero- and viral dynamics of PCV2 from 12 PCV2-contaminated pig herds with vaccination against PCV2 in Southern and Central Taiwan. In particular, the level of PCV2 load during the window period for seroconversion using real-time polymerase chain reaction and a commercial enzyme-linked immunosorbent assay (ELISA) kit. Our results revealed that pig herds showed slight or no seroconversion after three to four weeks post-PCV2 immunization. The presence of PCV2 was observed during the window period for seroconversion in all herds. In conclusion, natural exposure of PCV2 occurs in the growing to fattening period, and viremia can last until slaughter. Additionally, our findings indicate that using ELISA showed the level of antibodies and aided in the understanding and surveillance of the current PCV2 status in the field.
猪圆环病毒相关疾病（PCVADs）给养猪业造成了巨大的经济损失。为了尽量减少PCVAD带来的经济损失，人们研发了猪圆环病毒2型（PCV2）疫苗，如今在全球范围内广泛接种。然而，关于亚洲大陆养猪场中PCV2感染的现状，目前可获取的信息有限。本研究旨在评估台湾南部和中部12个接种过PCV2疫苗且受PCV2污染的猪群中PCV2的血清学和病毒动态。特别是，使用实时聚合酶链反应和商用酶联免疫吸附测定（ELISA）试剂盒检测血清转化窗口期的PCV2载量水平。我们的结果显示，在PCV2免疫接种后三到四周，猪群的血清转化轻微或未发生。在所有猪群的血清转化窗口期均检测到了PCV2的存在。总之，PCV2在生长育肥期自然感染，病毒血症可持续至屠宰。此外，我们的研究结果表明，ELISA检测有助于了解和监测猪场当前PCV2的感染状况。
