Shaaban Mona, Abd El-Rahman Ola A, Al-Qaidi Bashair, Ashour Hossam M
Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Department of Microbiology and Immunology, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo 11651, Egypt.
Microorganisms. 2020 Jun 26;8(6):960. doi: 10.3390/microorganisms8060960.
The emergence of biofilm-forming, multi-drug-resistant (MDR) infections is a serious threat that necessitates non-antibiotic therapies. Antibiotic susceptibility and biofilm-forming activity of isolates from urine samples were assessed by disc diffusion and crystal violet assays, respectively. Antimicrobial activities of probiotic by crystal violet assays. While most isolates were antibiotic-resistant to varying degrees, isolate P14 was MDR (resistant to ceftazidime, cefotaxime, amoxicillin-clavulanic acid, imipenem, ciprofloxacin, and amikacin) and formed strong biofilms. Cultures and cell-free supernatants of and exhibited antimicrobial and antibiofilm activities. The 1/16 concentration of untreated supernatants of and significantly reduced mature biofilm formation and adherence of P14 by 60% and 72%, respectively (for ), and by 73% each (for ). The 1/8 concentration of pH-adjusted supernatants of and significantly reduced mature biofilm formation and adherence of P14 by 39% and 75%, respectively (for ), and by 73% each (for ). Scanning electron microscopy (SEM) confirmed eradication of P14's biofilm by . and could be utilized to combat -associated urinary tract infections.
形成生物膜的多重耐药（MDR）感染的出现是一个严重威胁，这使得非抗生素疗法成为必要。分别通过纸片扩散法和结晶紫测定法评估尿样分离株的抗生素敏感性和生物膜形成活性。通过结晶紫测定法评估益生菌的抗菌活性。虽然大多数分离株都有不同程度的抗生素耐药性，但分离株P14是多重耐药的（对头孢他啶、头孢噻肟、阿莫西林-克拉维酸、亚胺培南、环丙沙星和阿米卡星耐药）且形成强生物膜。[具体益生菌名称]的培养物和无细胞上清液表现出抗菌和抗生物膜活性。[具体益生菌名称]未处理上清液的1/16浓度分别使P14的成熟生物膜形成和黏附显著降低60%和72%（对于[具体情况1]），以及各降低73%（对于[具体情况2]）。[具体益生菌名称]pH调节后上清液的1/8浓度分别使P14的成熟生物膜形成和黏附显著降低39%和75%（对于[具体情况1]），以及各降低73%（对于[具体情况2]）。扫描电子显微镜（SEM）证实[具体益生菌名称]可根除P14的生物膜。[具体益生菌名称]可用于对抗[相关情况]引起的尿路感染。
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