Liu Lijuan, Dong Zhiyou, Ai Shengquan, Chen Shanyu, Dong Mengyao, Li Qianlan, Zhou Ziyao, Liu Haifeng, Zhong Zhijun, Ma Xiaoping, Hu Yanchun, Ren Zhihua, Fu Hualin, Shu Gang, Qiu Xianmeng, Peng Guangneng
Key Laboratory of Animal Disease and Human Health of Sichuan, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, China.
New Ruipeng Pet Healthcare Group, Chengdu, China.
Front Microbiol. 2023 May 10;14:1141418. doi: 10.3389/fmicb.2023.1141418. eCollection 2023.
is a multi-host pathogen that causes diseases of varying severity in a wide range of mammals, including humans. is resistant to multiple antibiotics and has acquired the ability to produce expanded spectrum of β-lactamases, leading to serious public health problems. However, the available information on isolated from feces of dogs, is still poorly understood, as is the correlation between its virulence-associated genes (VAGs) and antibiotic resistance genes (ARGs).
In this study, we isolated 75 strains of from 241 samples, and investigated the swarming motility, biofilm formation, antimicrobial resistance (AMR), distribution of VAGs and ARGs, as well as the presence of class 1, 2, and 3 integrons in these isolates.
Our findings suggest a high prevalence of intensive swarming motility and strong biofilm formation ability among isolates. Isolates were primarily resistant to cefazolin (70.67%) and imipenem (70.67%). These isolates were found to carry , , , , , , , , , , and with varying prevalence levels of 100.00, 100.00, 100.00, 98.67, 98.67, 90.67, 90.67, 90.67, 90.67, 89.33, and 70.67%, respectively. Additionally, the isolates were found to carry , , , , , , , , and with varying prevalence levels of 38.67, 32.00, 25.33, 17.33, 16.00, 10.67, 5.33, 2.67, 1.33, and 1.33%, respectively. Among 40 MDR strains, 14 (35.00%) were found to carry class 1 integrons, 12 (30.00%) strains carried class 2 integrons, while no class 3 integrons was detected. There was a significant positive correlation between the class 1 integrons and three ARGs: , , and . This study revealed that strains isolated from domestic dogs exhibited a higher prevalence of MDR, and carried fewer VAGs but more ARGs compared to those isolated from stay dogs. Furthermore, a negative correlation was observed between VAGs and ARGs.
Given the increasing antimicrobial resistance of , veterinarians should adopt a prudent approach towards antibiotics administration in dogs to mitigate the emergence and dissemination of MDR strains that pose a potential threat to public health.
[病原体名称]是一种多宿主病原体，可在包括人类在内的多种哺乳动物中引起严重程度各异的疾病。[病原体名称]对多种抗生素具有抗性，并获得了产生超广谱β-内酰胺酶的能力，从而导致严重的公共卫生问题。然而，关于从狗粪便中分离出的[病原体名称]的现有信息仍然了解不足，其毒力相关基因（VAGs）与抗生素抗性基因（ARGs）之间的相关性也是如此。
在本研究中，我们从241份样本中分离出75株[病原体名称]，并研究了这些分离株的群集运动性、生物膜形成、抗菌药物耐药性（AMR）、VAGs和ARGs的分布，以及1类、2类和3类整合子的存在情况。
我们的研究结果表明，[病原体名称]分离株中群集运动性强烈和生物膜形成能力强的情况普遍存在。分离株主要对头孢唑林（70.67%）和亚胺培南（70.67%）耐药。发现这些分离株携带[基因名称1]、[基因名称2]、[基因名称3]、[基因名称4]、[基因名称5]、[基因名称6]、[基因名称7]、[基因名称8]、[基因名称9]、[基因名称10]和[基因名称11]，其流行率分别为100.00%、100.00%、100.00%、98.67%、98.67%、90.67%、90.67%、90.67%、90.67%、89.33%和70.67%。此外，还发现分离株携带[基因名称12]、[基因名称13]、[基因名称14]、[基因名称15]、[基因名称16]、[基因名称17]、[基因名称18]、[基因名称19]、[基因名称20]和[基因名称21]，其流行率分别为38.67%、32.00%、25.33%、17.33%、16.00%、10.67%、5.33%、2.67%、1.33%和1.33%。在40株多重耐药菌株中，发现14株（35.00%）携带1类整合子，12株（30.00%）携带2类整合子，未检测到3类整合子。1类整合子与三种ARGs（[基因名称22]、[基因名称23]和[基因名称24]）之间存在显著正相关。本研究表明，与从流浪狗分离出的菌株相比，从家犬分离出的[病原体名称]菌株多重耐药的流行率更高，携带的VAGs更少但ARGs更多。此外，还观察到VAGs与ARGs之间存在负相关。
鉴于[病原体名称]的抗菌药物耐药性不断增加，兽医在给狗使用抗生素时应采取谨慎的方法，以减少对公共卫生构成潜在威胁的多重耐药菌株的出现和传播。
