Tiradentes University (UNIT), 300, Murilo Dantas Ave, Farolândia 49032-490, Brazil.
Technology and Research Institute (ITP), 300, Murilo Dantas Ave, Farolândia 49032-490, Brazil.
Int J Mol Sci. 2020 Jun 26;21(12):4557. doi: 10.3390/ijms21124557.
Multi-walled carbon nanotubes functionalized with naringenin have been developed as new drug carriers to improve the performance of lung cancer treatment. The nanocarrier was characterized by Transmission Electron Microscopy (TEM), Fourier-Transform Infrared Spectroscopy (FTIR), X-ray photoelectron spectroscopy, Raman Spectroscopy, and Differential Scanning Calorimetry (DSC). Drug release rates were determined in vitro by the dialysis method. The cytotoxic profile was evaluated using the MTT assay, against a human skin () as a model for normal cells, and against an adenocarcinomic human alveolar basal epithelial (A569) cell line as a lung cancer in vitro model. The results demonstrated that the functionalization of carbon nanotubes with naringenin occurred by non-covalent interactions. The release profiles demonstrated a pH-responsive behavior, showing a prolonged release in the tumor pH environment. The naringenin-functionalized carbon nanotubes showed lower cytotoxicity on non-malignant cells (hFB) than free naringenin, with an improved anticancer effect on malignant lung cells (A549) as an in vitro model of lung cancer.
用柚皮素功能化的多壁碳纳米管已被开发为新型药物载体，以提高肺癌治疗的效果。该纳米载体通过透射电子显微镜（TEM）、傅里叶变换红外光谱（FTIR）、X 射线光电子能谱、拉曼光谱和差示扫描量热法（DSC）进行了表征。通过透析法在体外测定了药物释放率。通过 MTT 测定法评估细胞毒性谱，以人类皮肤 () 作为正常细胞的模型，以及以腺癌细胞人类肺泡基底上皮 (A569) 细胞系作为肺癌的体外模型。结果表明，柚皮素通过非共价相互作用对碳纳米管进行了功能化。释放曲线表现出 pH 响应行为，在肿瘤 pH 环境中显示出延长的释放。与游离柚皮素相比，柚皮素功能化碳纳米管对非恶性细胞 (hFB) 的细胞毒性较低，对恶性肺细胞 (A549) 具有更好的抗癌效果，作为肺癌的体外模型。
