Singh Neha, Sachdev Abhay, Gopinath P
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Nanobiotechnology Laboratory, Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
J Nanosci Nanotechnol. 2018 Mar 1;18(3):1534-1541. doi: 10.1166/jnn.2018.14222.
Nanoscale drug delivery systems have emerged as promising alternatives to overcome the problems associated with by conventional chemotherapy for cancer treatment such as poor drug stability and bio-distribution. Herein, we report a single walled carbon nanotubes (SWCNTs) based drug delivery system functionalized with polysaccharides such as alginate (ALG) and chitosan (CHI), which can be loaded with an anticancer drug curcumin (CUR). Modification of SWCNTs renders high drug loading efficiency and sustained drug release, imperative for drug activity. These were characterized through various tools viz, microscopic (transmission electron microscopy, scanning electron microscopy and atomic force microscopy) and zeta potential analysis. Incorporation of CUR inside the modified SWCNTs was studied through Fourier transform infrared spectroscopy, fluorescence and UV-visible spectroscopy. In vitro release studies were conducted to gain an insight into the pH-dependent release behavior of the entrapped CUR from modified SWCNTs. The anti-cancer potential was further demonstrated using human lung adenocarcinoma (A549) cells as a model system. Various cell culture based assays were performed to study the ability of released CUR from modified SWCNTs for inhibiting the cancer cell proliferation by inducing apoptosis.
纳米级药物递送系统已成为一种有前景的替代方案，以克服传统癌症化疗所带来的问题，如药物稳定性差和生物分布不佳等。在此，我们报道了一种基于单壁碳纳米管（SWCNTs）的药物递送系统，该系统用藻酸盐（ALG）和壳聚糖（CHI）等多糖进行功能化修饰，可负载抗癌药物姜黄素（CUR）。SWCNTs的修饰实现了高载药效率和药物的持续释放，这对药物活性至关重要。通过各种工具对其进行了表征，即显微镜（透射电子显微镜、扫描电子显微镜和原子力显微镜）和zeta电位分析。通过傅里叶变换红外光谱、荧光光谱和紫外可见光谱研究了CUR在修饰后的SWCNTs中的掺入情况。进行了体外释放研究，以深入了解修饰后的SWCNTs中包封的CUR的pH依赖性释放行为。以人肺腺癌（A549）细胞为模型系统进一步证明了其抗癌潜力。进行了各种基于细胞培养的试验，以研究修饰后的SWCNTs释放的CUR通过诱导凋亡抑制癌细胞增殖的能力。
