Department of Operative Gynaecology and Oncological Gynaecology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland.
Laboratory of Cancer Genetics, Department of Pathology, Polish Mother's Memorial Hospital Research Institute, Lodz, Poland
In Vivo. 2020 Jul-Aug;34(4):1765-1771. doi: 10.21873/invivo.11970.
The analysis of oestrogen receptor (ESR2) and cytochrome P450 family 19 subfamily A member (CYP19A1) gene expression in the context of the risk for endometriosis development.
Tissue specimens, collected from patients with endometriosis (n=100) and from control patients (n=100) embedded into paraffin blocks, provided the material for genetic studies, oriented towards the expression of ESR2 and CYP19A1 genes. The gene expression was assessed by the reverse transcription-polymerase chain reaction technique.
Higher expression levels of ESR2 gene were demonstrated in the patients with endometriosis in comparison with the healthy controls. The expression intensity of CYP19A1 gene was associated with endometriosis, manifested as abdominal wall nodules. A relationship was observed between CYP19A1 gene expression and the Revised American Society for Reproductive Medicine classification in the group with ovarian endometrioid cysts, as well as in the group with peritoneal endometriosis.
This study suggests the significant role of ESR2 and CYP19A1 gene expression in the pathogenesis of endometriosis.
分析雌激素受体（ESR2）和细胞色素 P450 家族 19 亚家族 A 成员（CYP19A1）基因表达与子宫内膜异位症发展风险的关系。
从子宫内膜异位症患者（n=100）和对照患者（n=100）的石蜡包埋组织标本中获取材料，进行基因研究，评估 ESR2 和 CYP19A1 基因的表达。采用逆转录-聚合酶链反应技术评估基因表达。
与健康对照组相比，子宫内膜异位症患者的 ESR2 基因表达水平更高。CYP19A1 基因的表达强度与腹壁结节形式的子宫内膜异位症有关。在卵巢子宫内膜样囊肿组和腹膜子宫内膜异位症组中，观察到 CYP19A1 基因表达与修订后的美国生殖医学学会分类之间存在关系。
本研究提示 ESR2 和 CYP19A1 基因表达在子宫内膜异位症发病机制中具有重要作用。
