Li Yuwei, Li Guangyao, Chen Xia, Huang Hengliu, Liao Ling, Yuan Tao, Deng Shaoli
Department of Laboratory Medicine, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing 400042, People's Republic of China.
Department of Hepatobiliary Surgery, Daping Hospital, Army Medical University (Third Military Medical University), Chongqing 400042, People's Republic of China.
Onco Targets Ther. 2020 Jun 11;13:5455-5466. doi: 10.2147/OTT.S254455. eCollection 2020.
Long noncoding RNAs (lncRNAs) in body fluids have been considered as promising novel biomarkers for tumor-related diseases. The present study aimed to investigate the expression level of lncRNA NONHSAT053785 in serum and its correlation with clinical characteristics of hepatocellular carcinoma (HCC) patients.
The droplet digital PCR (ddPCR) was used to measure the serum levels of NONHSAT053785 in 112 HCC patients, 96 chronic hepatitis B (CHB) patients, and 99 healthy controls (HC). The correlation between NONHSAT053785 and clinical characteristics was analyzed by chi-square test and Spearman correlation test. The risk factors of intrahepatic metastasis (IM) were detected by univariate and multivariate analyses. Furthermore, the diagnostic value of NONHSAT053785 in HCC and its predictive ability in IM were evaluated by the receiver operating characteristic (ROC) curves.
The level of NONHSAT053785 was significantly increased in the serum of HCC patients and was higher in HCC patients with IM as compared to those without. Additionally, the expression level of NONHSAT053785 was significantly related to IM, Child-Pugh classification, and peripheral blood indicators such as liver metabolic enzymes and positively correlated to IM, Barcelona Clinic Liver Cancer (BCLC) staging, and some peripheral blood indicators. Furthermore, the serum NONHSAT053785 was indicated as an independent predictor for IM in the elderly, non-smoking, drinking, and tumor size ≥5 cm subjects. The area under the ROC curve (AUC) was 0.801 ( <0.0001) for diagnosis of HCC and 0.678 ( =0.0015) for predicting IM.
The increase in serum NONHSAT053785 levels was related to an increased risk of IM, and hence, may serve as a novel biomarker for the diagnosis of HCC and the prediction of IM.
体液中的长链非编码RNA（lncRNA）被认为是肿瘤相关疾病有前景的新型生物标志物。本研究旨在调查lncRNA NONHSAT053785在血清中的表达水平及其与肝细胞癌（HCC）患者临床特征的相关性。
采用液滴数字PCR（ddPCR）检测112例HCC患者、96例慢性乙型肝炎（CHB）患者和99例健康对照（HC）血清中NONHSAT053785的水平。通过卡方检验和Spearman相关性检验分析NONHSAT053785与临床特征之间的相关性。通过单因素和多因素分析检测肝内转移（IM）的危险因素。此外，通过受试者工作特征（ROC）曲线评估NONHSAT053785在HCC诊断中的价值及其对IM的预测能力。
HCC患者血清中NONHSAT053785水平显著升高，与无IM的HCC患者相比，有IM的HCC患者该水平更高。此外，NONHSAT053785的表达水平与IM、Child-Pugh分级以及肝代谢酶等外周血指标显著相关，且与IM、巴塞罗那临床肝癌（BCLC）分期和一些外周血指标呈正相关。此外，血清NONHSAT053785被表明是老年、不吸烟、饮酒且肿瘤大小≥5 cm受试者IM的独立预测因子。诊断HCC的ROC曲线下面积（AUC）为0.801（<0.0001），预测IM的AUC为0.678（=0.0015）。
血清NONHSAT053785水平升高与IM风险增加相关，因此，可作为HCC诊断和IM预测的新型生物标志物。
