Department of Geriatrics, General Hospital of The Yangtze River Shipping, Wuhan Brain Hospital, Wuhan, Hubei, China.
Department of Gastroenterology, Affiliated Hospital of Jianghan University, Wuhan Sixth Hospital, Wuhan, Hubei, China.
Braz J Med Biol Res. 2021 Oct 29;54(12):e11459. doi: 10.1590/1414-431X2021e11459. eCollection 2021.
Reportedly, circular RNAs (circRNAs) are crucial regulators in cancer progression. Nonetheless, the molecular mechanism of circRNAs in hepatocellular carcinoma (HCC) has not been fully clarified. Gene expression omnibus (GEO) database was employed to screen out the differentially expressed circRNAs in HCC. qRT-PCR and western blot were executed to detect circ_0001806 expression, miR-193a-5p expression, and MMP16 mRNA and protein expressions in HCC. The effect of circ_0001806 on HCC was analyzed by the CCK-8 method and Transwell experiment. RIP assay, pull-down experiment, and dual-luciferase reporter gene experiment were applied to validate the targeting relationships among circ_0001806, miR-193a-5p, and MMP16. Circ_0001806 was up-modulated in HCC tissues and cell lines. Knockdown of circ_0001806 impeded the multiplication, migration, and invasion of HCC cells. Circ_0001806 could up-regulate MMP16 expression through repressing miR-193a-5p, thereby facilitating the malignant biological behaviors of HCC. Circ_0001806 promoted HCC progression by regulating miR-193a-5p/MMP16 axis.
据报道，环状 RNA（circRNAs）是癌症进展中的重要调控因子。然而，circRNAs 在肝细胞癌（HCC）中的分子机制尚未完全阐明。本研究利用基因表达综合数据库（GEO）筛选 HCC 中差异表达的 circRNAs。采用 qRT-PCR 和 Western blot 检测 HCC 中 circ_0001806 的表达、miR-193a-5p 的表达以及 MMP16 mRNA 和蛋白的表达。通过 CCK-8 法和 Transwell 实验分析 circ_0001806 对 HCC 的影响。采用 RIP assay、pull-down 实验和双荧光素酶报告基因实验验证 circ_0001806、miR-193a-5p 和 MMP16 之间的靶向关系。Circ_0001806 在 HCC 组织和细胞系中呈上调表达。circ_0001806 敲低可抑制 HCC 细胞的增殖、迁移和侵袭。Circ_0001806 通过抑制 miR-193a-5p 而上调 MMP16 表达，从而促进 HCC 的恶性生物学行为。Circ_0001806 通过调节 miR-193a-5p/MMP16 轴促进 HCC 进展。
