Shan Qiuli, Qu Fan, Yang Weiping, Chen Ningning
College of Biological Science and Technology, University of Jinan, Jinan 250022, People's Republic of China.
Department of Thyroid Breast Surgery, Qingdao Chengyang People's Hospital, Qingdao, People's Republic of China.
Cancer Manag Res. 2020 Jun 15;12:4561-4571. doi: 10.2147/CMAR.S249576. eCollection 2020.
To investigate the effect of LINC00657 on breast carcinoma by regulating miR-590-3p.
Ninety-seven cases with breast carcinoma who were admitted to Qingdao Chengyang People's Hospital were collected. The breast carcinoma (n=97) and tumor-adjacent tissues (n=97) of patients were collected during the operation with the permission of the patients. The expressions of LINC00657 and miR-590-3p were detected in breast carcinoma cells and tissues. The breast carcinoma cells were transfected and their proliferation, migration, invasion and apoptosis were detected.
LINC00657 was highly expressed in breast carcinoma tissues, while miR-590-3p was reduced (P<0.05). The proliferation, invasion and migration of cells transfected with si-LINC00657 or miR-590-3p-mimics were significantly inhibited, and the apoptosis rate increased, resulting in the up-regulation of the expressions of apoptosis-related proteins Bax and Caspase-3 and the reduction of Bcl-2 (P<0.05). After si-LINC00657 or miR-590-3p-mimics, the level of GOLPH3 decreased. Through double luciferase report and RIP experiment, it was confirmed that LINC00657 could act as a sponge of miR-590-3p to negatively regulate its expression. After correlation analysis, it was concluded that there was a negative correlation between LINC00657 and miR-590-3p. Rescue experiments concluded that co-transfection of si-LINC00657+miR-590-3P-inhibitor could reverse the inhibitory action of si-LINC00657 on breast carcinoma cells.
LINC00657 can participate in the biological behavior process of breast carcinoma by regulating miR-590-3p/GOLPH3 signal.
通过调控miR-590-3p来研究LINC00657对乳腺癌的影响。
收集青岛市城阳区人民医院收治的97例乳腺癌患者。在患者同意的情况下，于手术过程中收集患者的乳腺癌组织（n = 97）和癌旁组织（n = 97）。检测乳腺癌细胞和组织中LINC00657和miR-590-3p的表达。对乳腺癌细胞进行转染，并检测其增殖、迁移、侵袭和凋亡情况。
LINC00657在乳腺癌组织中高表达，而miR-590-3p表达降低（P<0.05）。转染si-LINC00657或miR-590-3p模拟物的细胞增殖、侵袭和迁移明显受到抑制，凋亡率增加，导致凋亡相关蛋白Bax和Caspase-3表达上调，Bcl-2表达降低（P<0.05）。转染si-LINC00657或miR-590-3p模拟物后，GOLPH3水平降低。通过双荧光素酶报告和RIP实验证实，LINC00657可作为miR-590-3p的海绵，负向调控其表达。相关性分析得出，LINC00657与miR-590-3p呈负相关。挽救实验得出，共转染si-LINC00657+miR-590-3P抑制剂可逆转si-LINC00657对乳腺癌细胞的抑制作用。
LINC00657可通过调控miR-590-3p/GOLPH3信号参与乳腺癌的生物学行为过程。
