Sauder D N, Stanulis-Praeger B M, Gilchrest B A
Department of Medicine, McMaster University, Hamilton, Canada.
Arch Dermatol Res. 1988;280(2):71-6. doi: 10.1007/BF00417707.
The monocyte-derived cytokine interleukin-1 (IL-1) has growth-promoting activity for a variety of cell types, including lymphocytes and fibroblasts. We have previously shown that the epidermal cell-derived thymocyte-activating factor (ETAF) strongly resembles IL-1 in terms of biological, biochemical, and molecular biological properties. Because some lymphokines are known ot alter epidermal cell growth and differentiation and because cultured epidermal keratinocytes are capable of autocrine growth stimulation in vitro through "conditioning" of their culture medium, we sought to evaluate the effect of ETAF on keratinocyte growth. While there was marked donor variability in the responsiveness of keratinocytes to ETAF, partially purified preparations of ETAF showed substantial ability to stimulate the growth of keratinocytes, particularly those of newborn donors. In addition, in conditioned media there appeared to be activities distinct from ETAF that also promoted keratinocyte growth. Keratinocytes in serum-free medium secreted large amounts of ETAF, as reported previously, and keratinocyte cultures derived from newborn donors secreted significantly more than did those derived from adult donors. These results are consistent with an autocrine growth regulatory role of ETAF in human epidermis and with an age-associated loss of this phenomenon.
单核细胞衍生的细胞因子白细胞介素-1（IL-1）对多种细胞类型具有促生长活性，包括淋巴细胞和成纤维细胞。我们之前已经表明，表皮细胞衍生的胸腺细胞激活因子（ETAF）在生物学、生物化学和分子生物学特性方面与IL-1极为相似。由于已知一些淋巴因子会改变表皮细胞的生长和分化，并且由于培养的表皮角质形成细胞能够通过其培养基的“条件化”在体外进行自分泌生长刺激，我们试图评估ETAF对角质形成细胞生长的影响。虽然角质形成细胞对ETAF的反应存在明显的供体差异，但部分纯化的ETAF制剂显示出显著的刺激角质形成细胞生长的能力，尤其是对新生儿供体的角质形成细胞。此外，在条件培养基中似乎存在与ETAF不同的、也能促进角质形成细胞生长的活性物质。如先前报道的那样，无血清培养基中的角质形成细胞分泌大量的ETAF，并且来自新生儿供体的角质形成细胞培养物分泌的ETAF明显多于来自成人供体的培养物。这些结果与ETAF在人表皮中的自分泌生长调节作用以及这种现象与年龄相关的丧失是一致的。
