Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University-Seoul, Goyang, 10326, Republic of Korea.
Department of Agricultural Biotechnology and Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Probiotics Antimicrob Proteins. 2021 Feb;13(1):72-79. doi: 10.1007/s12602-020-09682-3.
Probiotics in livestock feed supplements are considered to be an alternative to antibiotics. However, effector molecules responsible for the beneficial roles of probiotics in pigs are in general not well known. Thus, this study demonstrated that a well-known virulence factor, flagellin of Salmonella typhimurium, significantly induced IL-8 production in porcine peripheral blood mononuclear cells, whereas lipoteichoic acid (LTA), a major cell wall component of Gram-positive bacteria Lactobacillus plantarum, L. casei, and L. rhamnosus GG, effectively inhibited flagellin-induced IL-8 production at mRNA and protein levels. However, the lipoproteins of L. plantarum, L. casei, and L. rhamnosus GG did not suppress flagellin-induced IL-8 production. While D-alanine-deficient L. plantarum LTA inhibited flagellin-induced IL-8 production, L. plantarum LTA deficient in both D-alanine and acyl chains failed to inhibit it; this suggests that the acyl moieties of L. plantarum LTA are essential for inhibiting flagellin-induced IL-8 production. Taken together, L. plantarum LTA plays an important role in improving anti-inflammatory responses of porcine peripheral blood mononuclear cells.
在牲畜饲料补充剂中使用益生菌被认为是抗生素的替代品。然而，益生菌在猪中发挥有益作用的效应分子通常并不为人所知。因此，本研究表明，一种众所周知的毒力因子——鼠伤寒沙门氏菌的鞭毛蛋白，可显著诱导猪外周血单核细胞产生 IL-8，而脂磷壁酸（LTA），革兰氏阳性菌乳酸乳球菌、干酪乳杆菌和鼠李糖乳杆菌 GG 的主要细胞壁成分，可有效抑制鞭毛蛋白诱导的 IL-8 在 mRNA 和蛋白水平上的产生。然而，乳酸乳球菌、干酪乳杆菌和鼠李糖乳杆菌 GG 的脂蛋白并不能抑制鞭毛蛋白诱导的 IL-8 产生。虽然 D-丙氨酸缺陷型乳酸乳球菌 LTA 抑制鞭毛蛋白诱导的 IL-8 产生，但既缺乏 D-丙氨酸又缺乏酰基链的乳酸乳球菌 LTA 则不能抑制其产生；这表明乳酸乳球菌 LTA 的酰基部分对于抑制鞭毛蛋白诱导的 IL-8 产生是必需的。综上所述，乳酸乳球菌 LTA 在外周血单核细胞抗炎反应的改善中发挥重要作用。
