Bergeron Nadia, Corriveau Jonathan, Letellier Ann, Daigle France, Quessy Sylvain
Chaire de recherche en salubrité des viandes, Groupe de recherche sur les maladies infectieuses du porc, Département de pathologie et microbiologie, Faculté de médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
Can J Vet Res. 2010 Jan;74(1):11-7.
Salmonella Typhimurium is frequently isolated from pigs and may also cause enteric disease in humans. In this study, 33 isolates of S. Typhimurium associated with septicemia in swine (CS) were compared to 33 isolates recovered from healthy animals at slaughter (WCS). The isolates were characterized using phenotyping and genotyping methods. For each isolate, the phage type, antimicrobial resistance, and pulsed-field gel electrophoresis (PFGE) DNA profiles were determined. In addition, the protein profiles of each isolate grown in different conditions were studied by Coomassie Blue-stained sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblot. Various phage types were identified. The phage type PT 104 represented 36.4% of all isolates from septicemic pigs. Resistance to as many as 12 antimicrobial agents, including some natural resistances, was found in isolates from CS and WCS. Many genetic profiles were identified among the PT 104 phage types. Although it was not possible to associate one particular protein with septicemic isolates, several highly immunogenic proteins, present in all virulent isolates and in most isolates from clinically healthy animals, were identified. These results indicated that strains associated with septicemia belong to various genetic lineages that can also be recovered from asymptomatic animals at the time of slaughter.
鼠伤寒沙门氏菌经常从猪身上分离出来，也可能导致人类肠道疾病。在本研究中，将33株与猪败血症（CS）相关的鼠伤寒沙门氏菌分离株与33株从屠宰时的健康动物中分离出的菌株（WCS）进行了比较。使用表型和基因分型方法对这些分离株进行了鉴定。对于每一株分离株，确定了噬菌体类型、抗菌药物耐药性和脉冲场凝胶电泳（PFGE）DNA图谱。此外，通过考马斯亮蓝染色的十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹研究了在不同条件下生长的每一株分离株的蛋白质图谱。鉴定出了多种噬菌体类型。噬菌体类型PT 104占败血症猪所有分离株的36.4%。在CS和WCS的分离株中发现了对多达12种抗菌药物的耐药性，包括一些天然耐药性。在PT 104噬菌体类型中鉴定出了许多基因图谱。虽然不可能将一种特定的蛋白质与败血症分离株联系起来，但鉴定出了几种在所有强毒株和大多数临床健康动物的分离株中都存在的高免疫原性蛋白质。这些结果表明，与败血症相关的菌株属于各种遗传谱系，这些谱系在屠宰时也可以从无症状动物中分离出来。
