Departamento de Sanidad Vegetal, Facultad de Ciencias Agronómicas, Universidad de Chile, Santiago, Chile.
Plant Dis. 2020 Sep;104(9):2324-2329. doi: 10.1094/PDIS-09-19-2055-SC. Epub 2020 Jun 30.
Table grapes are highly susceptible to infections during the bloom period. After reaching the flower development stage, remains quiescent until berry ripening or gives rise to blossom blight under specific climate conditions. A research study was conducted on the Chilean Central Valley during the 2018-2019 growing season. Flowers of cv. Thompson Seedless were collected and was isolated together to a second and morphologically different species, characterized by white mycelium and low to no sporulation (11.4% of total isolates). Three randomly selected isolates within this population were genetically examined and identified as based on a phylogenetic multilocus approach using partial regions of genes , , and or and . Pathogenicity tests showed that infects and causes wilting in healthy table grape flowers. isolates were able to infect Thompson Seedless berries, inducing lesions between 13.11 and 41.53% with respect to the lesion diameter generated by B05.10. The fungicide sensitivity was evaluated. The three genetically characterized isolates were sensitive to boscalid and to cyprodinil/fludioxonil mixture with a mean EC value of 5.5 µg/ml and 0.065 µg/ml, respectively. However, loss of sensitivity to fenhexamid was determined, with a mean EC value of 5.13 µg/ml. Our understanding about blossom blight in has been limited to . Here we associated as a second causal agent of this disease in Chile. This data represents a first approach to the epidemiological characteristics of associated with blossom blight in table grapes.
鲜食葡萄在开花期极易受到 感染。在达到花发育阶段后， 处于休眠状态，直到在特定气候条件下浆果成熟或引发花腐病。在 2018-2019 生长季节对智利中央山谷进行了一项研究。采集了 cv. Thompson Seedless 的花朵，并分离出第二种形态不同的物种，其特征为白色菌丝和低至无孢子形成（总分离物的 11.4%）。从该种群中随机选择了三个分离物进行遗传检查，并根据基于基因 、 、 和 或 和 的部分区域的系统发育多基因方法鉴定为 。致病性测试表明， 感染并导致健康鲜食葡萄花朵萎蔫。 分离物能够感染 Thompson Seedless 浆果，引起的病变分别为 13.11%至 41.53%，病变直径由 B05.10 产生。评估了杀菌剂的敏感性。三种遗传特征明确的分离物对 boscalid 和 cyprodinil/fludioxonil 混合物敏感，平均 EC 值分别为 5.5 µg/ml 和 0.065 µg/ml。然而，确定对 fenhexamid 的敏感性丧失，平均 EC 值为 5.13 µg/ml。我们对 中花腐病的了解仅限于 。在这里，我们将 作为该疾病在智利的第二种病原体。这代表了与鲜食葡萄花腐病相关的 与花腐病相关的流行病学特征的首次尝试。
