Saito S, Margosan D, Michailides T J, Xiao C L
United States Department of Agriculture, Agricultural Research Service (USDA-ARS), San Joaquin Valley Agricultural Sciences Center, Parlier, California 93648.
USDA-ARS, San Joaquin Valley Agricultural Sciences Center, Parlier, California 93648.
Mycologia. 2016 Mar-Apr;108(2):330-43. doi: 10.3852/15-165. Epub 2016 Jan 6.
The Botrytis cinerea species complex comprises two cryptic species, originally referred to Group I and Group II based on Bc-hch gene RFLP haplotyping. Group I was described as a new cryptic species B. pseudocinerea During a survey of Botrytis spp. causing gray mold in blueberries and table grapes in the Central Valley of California, six isolates, three from blueberries and three from table grapes, were placed in Group I but had a distinct morphological character with conidiophores significantly longer than those of B. cinerea and B. pseudocinerea We compared these with B. cinerea and B. pseudocinerea by examining morphological and physiological characters, sensitivity to fenhexamid and phylogenetic analysis inferred from sequences of three nuclear genes. Phylogenetic analysis with the three partial gene sequences encoding glyceraldehyde-3-phosate dehydrogenase (G3PDH), heat-shock protein 60 (HSP60) and DNA-dependent RNA polymerase subunit II (RPB2) supported the proposal of a new Botrytis species, B. californica, which is closely related genetically to B. cinerea, B. pseudocinerea and B. sinoviticola, all known as causal agents of gray mold of grapes. Botrytis californica caused decay on blueberry and table grape fruit inoculated with the fungus. This study suggests that B. californica is a cryptic species sympatric with B. cinerea on blueberries and table grapes in California.
灰葡萄孢菌复合体包含两个隐性物种，最初根据Bc-hch基因限制性片段长度多态性单倍型分析分为第一组和第二组。第一组被描述为一个新的隐性物种——拟灰葡萄孢菌。在对加利福尼亚中央山谷蓝莓和鲜食葡萄上引起灰霉病的葡萄孢菌进行调查时，有六个分离株（三个来自蓝莓，三个来自鲜食葡萄）被归入第一组，但它们具有独特的形态特征，其分生孢子梗明显长于灰葡萄孢菌和拟灰葡萄孢菌。我们通过检查形态和生理特征、对苯菌酮的敏感性以及从三个核基因序列推断的系统发育分析，将这些分离株与灰葡萄孢菌和拟灰葡萄孢菌进行了比较。对编码甘油醛-3-磷酸脱氢酶（G3PDH）、热休克蛋白60（HSP60）和DNA依赖性RNA聚合酶亚基II（RPB2）的三个部分基因序列进行的系统发育分析支持了一个新的葡萄孢菌物种——加利福尼亚葡萄孢菌的提议，该物种在遗传上与灰葡萄孢菌、拟灰葡萄孢菌和中国葡萄孢菌密切相关，后三者均为已知的葡萄灰霉病病原体。加利福尼亚葡萄孢菌对接种该真菌的蓝莓和鲜食葡萄果实造成腐烂。这项研究表明，加利福尼亚葡萄孢菌是一种与加利福尼亚蓝莓和鲜食葡萄上的灰葡萄孢菌同域分布的隐性物种。
