Stene M A, Babajanians M, Bhuta S, Cochran A J
John Wayne Clinic, Armand Hammer Laboratories, Jonsson Comprehensive Cancer Center, School of Medicine, University of California, Los Angeles.
J Invest Dermatol. 1988 Aug;91(2):125-8. doi: 10.1111/1523-1747.ep12464142.
Melanomas are associated with a T-cell predominant infiltrate that may cause their regression. Langerhans cells (LC) are essential for initiation and maintenance of specific T-cell-mediate responses in the skin. Therefore, a change in this antigen-presenting LC population may alter the host response. To determine whether the LC population varies during the evolution of primary cutaneous melanoma 32 melanocytic lesions, nevi, and cutaneous melanomas were studied by quantitative immunohistology. The monoclonal antibody, Leu-6, and the avidin biotin complex immunoperoxidase method were used to identify LC. Compared with histologically normal melanoma-adjacent skin, epidermal LC were depleted above "deeply invasive" melanomas but were relatively unchanged above nevi, "early invasive" melanomas, and cutaneous metastatic melanoma nodules. Dermal LC were significantly increased around in situ and "early invasive" melanomas but not around "deeply invasive" melanomas or cutaneous metastatic nodules. Dermal LC are thus associated with early transformed melanocytes and may present neoantigens to T lymphocytes in situ or after LC maturation in the draining lymph node. Melanoma-associated LC decline in number as melanoma progresses.
黑色素瘤与以T细胞为主的浸润有关，这种浸润可能导致其消退。朗格汉斯细胞（LC）对于皮肤中特异性T细胞介导反应的启动和维持至关重要。因此，这种抗原呈递LC群体的变化可能会改变宿主反应。为了确定在原发性皮肤黑色素瘤演变过程中LC群体是否发生变化，通过定量免疫组织学研究了32个黑素细胞性病变、痣和皮肤黑色素瘤。使用单克隆抗体Leu-6和抗生物素蛋白-生物素复合物免疫过氧化物酶方法来识别LC。与组织学正常的黑色素瘤相邻皮肤相比，在“深度浸润性”黑色素瘤上方表皮LC减少，但在痣、“早期浸润性”黑色素瘤和皮肤转移性黑色素瘤结节上方相对未改变。真皮LC在原位和“早期浸润性”黑色素瘤周围显著增加，但在“深度浸润性”黑色素瘤或皮肤转移性结节周围未增加。因此，真皮LC与早期转化的黑素细胞有关，并且可能在原位或在引流淋巴结中LC成熟后将新抗原呈递给T淋巴细胞。随着黑色素瘤进展，与黑色素瘤相关的LC数量下降。
