Department of Orthopedic Surgery, Graded Implants and Regenerative Strategies, OE 8893, Hannover Medical School (MHH), 30625 Hannover, Germany.
Lower Saxony Centre for Biomedical Engineering, Implant Research and Development (NIFE), 30625 Hannover, Germany.
Cells. 2020 Jun 29;9(7):1580. doi: 10.3390/cells9071580.
Mesenchymal stromal cells (MSCs) are a promising cell source for tissue engineering and regenerative medicine. In our lab, we found that MSC preparations from bone marrow of many different donors had a limited capacity of in vitro differentiation into osteogenic and chondrogenic lineages-a capacity claimed to be inherent to MSCs. The current study was designed to test the hypothesis that the amount of heparin used as anticoagulant during bone marrow harvest had an inhibitory influence on the in vitro differentiation capacity of isolated MSCs. Bone marrow was obtained from the femoral cavity of twelve donors during total hip arthroplasty in the absence or presence of heparin. No coagulation was observed in the absence of heparin. The number of mononuclear cells was independent of heparin addition. Isolated MSCs were characterized by morphology, population doubling times, expression of cell surface antigens and in vitro differentiation. Results of these analyses were independent of the amount of heparin. Transcriptome analyses of cells from three randomly chosen donors and quantitative realtime PCR (qRT-PCR) analysis from cells of all donors demonstrated no clear effect of heparin on the transcriptome of the cells. This excludes heparin as a potential source of disparate results.
间充质基质细胞（MSCs）是组织工程和再生医学有前途的细胞来源。在我们的实验室中，我们发现来自许多不同供体的骨髓 MSC 制剂在体外向成骨和成软骨谱系分化的能力有限 - 据称这是 MSC 的固有能力。本研究旨在测试以下假设：在骨髓采集过程中用作抗凝剂的肝素用量对分离的 MSC 的体外分化能力具有抑制作用。在全髋关节置换术期间，从 12 名供体的股骨髓腔中获得骨髓，不存在或存在肝素。不存在肝素时观察不到凝血。单核细胞的数量与肝素的添加无关。分离的 MSC 通过形态，倍增时间，细胞表面抗原的表达和体外分化来表征。这些分析的结果与肝素的量无关。来自三个随机选择的供体的细胞的转录组分析和来自所有供体的细胞的定量实时 PCR（qRT-PCR）分析均表明肝素对细胞的转录组没有明显影响。这排除了肝素作为导致结果差异的潜在来源。
