Department of Biosciences, Molecular Cell Biology of Plants, Goethe University, 60438, Frankfurt am Main, Germany.
Buchmann Institute for Molecular Life Sciences, Goethe University, 60438, Frankfurt am Main, Germany.
Sci Rep. 2020 Jul 1;10(1):10694. doi: 10.1038/s41598-020-67833-6.
Cellular transitions during development and stress response depend on coordinated transcriptomic and proteomic alterations. Pollen is particular because its development is a complex process that includes meiotic and mitotic divisions which causes a high heat sensitivity of these cells. Development and stress response are accompanied by a reprogramming of the transcriptome, e.g. by post-transcriptional regulation via miRNAs. We identified known and potentially novel miRNAs in the transcriptome of developing and heat-stressed pollen of Solanum lycopersicum (tomato). The prediction of target mRNAs yielded an equal number of predicted target-sites in CDS and 3'UTR regions of target mRNAs. The result enabled the postulation of a possible link between miRNAs and a fine-tuning of transcription factor abundance during pollen development. miRNAs seem to play a role in the pollen heat stress response as well. We identified several heat stress transcription factors and heat shock proteins as putative targets of miRNAs in response to heat stress, thereby placing these miRNAs as important elements of thermotolerance. Moreover, for members of the AP2, SBP and ARF family members we could predict a miRNA-mediated regulation during development via the miR172, mir156 and mir160-family strengthening the current concept of a cross-connection between development and stress response in plants.
细胞在发育和应激反应过程中的转变取决于转录组和蛋白质组的协调变化。花粉是特殊的，因为它的发育是一个复杂的过程，包括减数分裂和有丝分裂，这导致这些细胞对热高度敏感。发育和应激反应伴随着转录组的重编程，例如通过 miRNA 进行转录后调控。我们在发育中的和受热胁迫的番茄花粉的转录组中鉴定了已知的和潜在的新的 miRNA。靶 mRNA 的预测在 CDS 和靶 mRNA 的 3'UTR 区域产生了相同数量的预测靶位。该结果使得可以假设 miRNA 与花粉发育过程中转录因子丰度的精细调控之间可能存在联系。miRNA 似乎在花粉热应激反应中也发挥作用。我们鉴定了几个热应激转录因子和热休克蛋白作为 miRNA 在热应激下的靶标，从而将这些 miRNA 作为耐热性的重要组成部分。此外，对于 AP2、SBP 和 ARF 家族成员，我们可以通过 miR172、mir156 和 mir160 家族来预测发育过程中的 miRNA 调节，从而加强了植物发育和应激反应之间交叉连接的现有概念。
