中国羊驼中 spp. 的分子鉴定。
Molecular identification of spp. in alpacas () in China.
作者信息
Zhang Qiyuan, Li Junqiang, Li Zhiguo, Xu Chunyan, Hou Minyu, Qi Meng
机构信息
College of Animal Science, Tarim University, Alar, Xinjiang, 843300, China.
Academy of Chinese Medical Sciences, Henan University of Chinese Medicine, Zhengzhou, 450046, China.
出版信息
Int J Parasitol Parasites Wildl. 2020 Jun 20;12:181-184. doi: 10.1016/j.ijppaw.2020.06.007. eCollection 2020 Aug.
is a ubiquitous protozoan in human and animals. To investigate the genetic diversity of spp. in alpaca () in China, 484 fecal samples from alpacas were collected at nine sites, and spp. were screened with PCR amplification of the small subunit ribosome RNA (SSU rRNA) locus. spp. infected 2.9% (14/484) of the alpacas. Of the nine collection sites, two were positive for , Wensu (3.0%, 3/100) and Qinghe (31.4%, 11/35). Three species were identified: (n = 2), (n = 1), and (n = 11). and were further subtyped with the 60-kDa glycoprotein locus (). The two isolates were subtype IIdA15G1, but the one isolate was not subtyped successfully. A phylogenetic analysis indicated that the isolates clustered with previously identified species. To our knowledge, this is the first report of infections in alpacas in China and provides baseline data for the study of in alpacas in China.
是一种在人类和动物中普遍存在的原生动物。为了调查中国 alpaca（羊驼）中 spp. 的遗传多样性，在九个地点采集了 484 份羊驼粪便样本，并通过小亚基核糖体 RNA（SSU rRNA）基因座的 PCR 扩增对 spp. 进行筛选。 spp. 感染了 2.9%（14/484）的羊驼。在九个采集地点中，有两个地点检测到 呈阳性，分别是温宿（3.0%，3/100）和青河（31.4%，11/35）。鉴定出了三种 物种： （n = 2）、 （n = 1）和 （n = 11）。 和 通过 60-kDa 糖蛋白基因座（）进一步分型。两个 分离株为 IIdA15G1 亚型，但一个 分离株未成功分型。系统发育分析表明， 分离株与先前鉴定的物种聚类。据我们所知，这是中国羊驼感染 的首次报告，并为中国羊驼 的研究提供了基线数据。
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