Stamford J A, Kruk Z L, Millar J
Department of Pharmacology, London Hospital Medical College, U.K.
Brain Res. 1988 Jun 28;454(1-2):282-8. doi: 10.1016/0006-8993(88)90828-1.
Fast cyclic voltammetry at carbon fibre microelectrodes was used to monitor stimulated release of electroactive species simultaneously in both caudate and nucleus accumbens. The released species was identified as dopamine in both nuclei on the basis of anatomical, electrochemical and pharmacological criteria. Dopamine release in the nucleus accumbens was consistently evoked at more ventromedial stimulation sites than for caudate. In both nuclei, release was reduced or abolished by alpha-methyl-p-tyrosine and Ro 4-1284. Pargyline, cocaine and GBR 12909 potentiated release while desipramine had no effect. In conjunction with the electrochemical data, the results indicate that dopamine is the sole species detected in caudate and accumbens following stimulation of the median forebrain bundle.
利用碳纤维微电极上的快速循环伏安法，同时监测尾状核和伏隔核中电活性物质的刺激释放。根据解剖学、电化学和药理学标准，确定两个核中释放的物质均为多巴胺。伏隔核中的多巴胺释放在比尾状核更腹内侧的刺激部位持续被诱发。在两个核中，α-甲基-对-酪氨酸和Ro 4-1284可减少或消除释放。帕吉林、可卡因和GBR 12909增强释放，而地昔帕明则无作用。结合电化学数据，结果表明，刺激中脑前束后，多巴胺是在尾状核和伏隔核中检测到的唯一物质。
