Miyama-Inaba M, Kuma S, Inaba K, Ogata H, Iwai H, Yasumizu R, Muramatsu S, Steinman R M, Ikehara S
First Department of Pathology, Kansai Medical University, Moriguchi, Japan.
J Exp Med. 1988 Aug 1;168(2):811-6. doi: 10.1084/jem.168.2.811.
A small number of B cells are found in the thymus of normal mice. A population of B lymphocytes could be enriched to greater than 90% purity by isolating a low-density fraction on Percoll density gradients and then depleting T cells with a mixture of anti-Thy-1, CD4, and CD8 mAbs and complement. Enrichment was monitored by surface Ig staining and by functional studies (responsiveness to LPS, and to anti-mu plus IL-4). When the phenotype of these B cells was studied by flow cytometry, 60-80% had the phenotype Ly-1+ (CD5), Ia+, B220low (CD45R), and Mac-1+ (CD 11b). In contrast, splenic B cells lacked CD5 and CD11b and expressed higher levels of B220 and Ia antigens. These results indicate that most thymic B cells have the phenotype of the Ly-1 B cell subset, which was identified previously as a trace subpopulation in some peripheral tissues and is thought to play a role in autoantibody formation.
在正常小鼠的胸腺中可发现少量B细胞。通过在Percoll密度梯度上分离低密度组分，然后用抗Thy-1、CD4和CD8单克隆抗体及补体混合物去除T细胞，可将一群B淋巴细胞富集至纯度大于90%。通过表面免疫球蛋白染色和功能研究（对LPS以及抗μ加IL-4的反应性）监测富集情况。当通过流式细胞术研究这些B细胞的表型时，60 - 80%具有Ly-1+(CD5)、Ia+、B220low(CD45R)和Mac-1+(CD11b)的表型。相比之下，脾B细胞缺乏CD5和CD11b，且表达更高水平的B220和Ia抗原。这些结果表明，大多数胸腺B细胞具有Ly-1 B细胞亚群的表型，该亚群先前在一些外周组织中被鉴定为微量亚群，并被认为在自身抗体形成中起作用。
