Kaper J B, Sayler G S, Baldini M M, Colwell R R
Appl Environ Microbiol. 1977 Apr;33(4):829-35. doi: 10.1128/aem.33.4.829-835.1977.
A primary, nonselective, ambient-temperature enrichment procedure for isolation of Salmonella spp. is described. The procedure was superior to elevated-temperature selective enrichment for Salmonella when estuarine water samples were examined. Five Chesapeake Bay stations were monitored, over an 8-month period, for the presence of salmonellae. Of 72 water and sediment samples collected, 17 (23.6%) yielded Salmonella spp. Seven serotypes were identified among the isolates. A seasonal pattern was noted for the incidence of the salmonellae. A most probable number procedure, performed by membrane filtration and nonselective enrichment, yielded Salmonella most probable number indices as high as 110 per 100 g of sediment. The results suggest that new methods, such as the one described in this report, are required for isolation of human intestinal pathogens from estuaries and coastal waters.
本文描述了一种用于分离沙门氏菌属的初级、非选择性、常温富集程序。当检测河口水样时，该程序在分离沙门氏菌方面优于高温选择性富集程序。在8个月的时间里，对切萨皮克湾的5个站点进行了监测，以检测沙门氏菌的存在。在采集的72份水和沉积物样本中，17份（23.6%）检出沙门氏菌属。在分离出的菌株中鉴定出了7种血清型。观察到沙门氏菌的发病率存在季节性模式。通过膜过滤和非选择性富集进行的最大可能数程序得出，每100克沉积物中沙门氏菌的最大可能数指数高达110。结果表明，需要新的方法，如本报告中所述的方法，来从河口和沿海水域分离人类肠道病原体。
