Altern Ther Health Med. 2021 Sep;27(5):33-37.
Acetylcholine (ACh) breaks down in a very short time in diseases related to memory loss. It's a neurotransmitter involved in cholinergic transmission in the brain. Acetylcholinesterase (AChE) hydrolyzes ACh. When AChE is inhibited, the ACh levels increase in the cholinergic synapses. The investigation of natural AChE inhibitors with minimal side effects has become important.
Objective • This study intended to determine the total phenolic content, total flavonoid contents, radical scavenging activities, and antiacetylcholinesterase activities of ethanolic extracts of carob pods (ceratonia siliqua), clove buds (eugenia aromatica), and linden flowers (tilia cordata).
Design • The research team designed an in-vitro study.
Setting • The study took place at a biochemistry research laboratory where purification of enzymes and studies on their kinetic properties and inhibitions are carried out.
Outcome measures • The antioxidant properties of the extracts including the total phenolic content (TPC), total flavonoid content (TFC), and free radical scavenging activities, were determined. The AChE enzyme was partially purified by DE-52 anion exchange chromatography from human erythrocytes. Besides, The AChE inhibitory properties of the ethanolic extracts were investigated.
Results • The TPCs of the carob pods, clove buds, and linden flowers were 46.78 ± 0.020, 103.57 ± 0.020, and 28.81 ± 0.031, mg GAE/L, respectively. The TFCs were 27.35 ± 0.021, 30.85 ± 0.017, and 32.12 ± 0.022 mg QE/L, respectively. While the extracts of carob pods and linden flowers inhibited AChE, with IC50s of 0.838 mg/ml and 0.156 mg/ml, respectively, clove buds didn't show inhibitory effect.
Conclusion • Although the clove buds had the maximum TPC; 1,1-diphenyl-2-picryl hydrazyl (DPPH); and 2,2'-azino-bis [3-ethylbenzothiazoline-6-sulphonic acid] (ABTS+) radical scavenging activity, it didn't show anticholinesterase activity.
乙酰胆碱（ACh）在与记忆丧失相关的疾病中会在很短的时间内分解。它是一种参与大脑胆碱能传递的神经递质。乙酰胆碱酯酶（AChE）水解 ACh。当 AChE 被抑制时，胆碱能突触中的 ACh 水平会增加。因此，寻找副作用最小的天然 AChE 抑制剂变得尤为重要。
本研究旨在测定角豆荚（Ceratonia siliqua）、丁香花蕾（Eugenia aromatica）和椴树花（Tilia cordata）的乙醇提取物的总酚含量、总黄酮含量、自由基清除活性和抗乙酰胆碱酯酶活性。
研究团队设计了一项体外研究。
研究在一个生物化学研究实验室进行，在那里进行酶的纯化以及对其动力学特性和抑制作用的研究。
角豆荚、丁香花蕾和椴树花的总酚含量（TPC）分别为 46.78±0.020、103.57±0.020 和 28.81±0.031mgGAE/L，总黄酮含量（TFC）分别为 27.35±0.021、30.85±0.017 和 32.12±0.022mgQE/L。角豆荚和椴树花的提取物抑制乙酰胆碱酯酶，其 IC50 分别为 0.838mg/ml 和 0.156mg/ml，而丁香花蕾则没有表现出抑制作用。
尽管丁香花蕾的总酚含量最高，具有 1,1-二苯基-2-苦基肼基（DPPH）和 2,2'-联氮-双（3-乙基苯并噻唑啉-6-磺酸）（ABTS+）自由基清除活性，但它没有表现出抗胆碱酯酶活性。
