Department of Bacteriology I, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku, Tokyo 162-8640, Japan.
Department of International Health and Medical Anthropology, Institute of Tropical Medicine, Nagasaki University, 1-12-4 Sakamoto, Nagasaki, Nagasaki 852-8523, Japan.
Infect Genet Evol. 2020 Nov;85:104447. doi: 10.1016/j.meegid.2020.104447. Epub 2020 Jun 30.
Leptospira spp. are the causative agents of leptospirosis, a zoonosis that occurs worldwide, and are maintained by various mammals. Recent molecular typing studies have indicated the existence of a specific Leptospira genotype-host association; however, few studies have shown the colonization of a single genotype in multiple animal genera. To gain further insights into the Leptospira genotype-host association, we conducted whole genome sequencing of 35 strains of L. borgpetersenii serogroup Javanica isolated from various Rattus species in Southern Japan, Philippines, and Taiwan. The SNP-based phylogenetic tree showed that the strains were grouped into five clusters based on their geographic location, indicating geographical structuring of genetic diversity in L. borgpetersenii serogroup Javanica strains. Furthermore, phylogenetic analysis indicated that the L. borgpetersenii serogroup Javanica can infect various animal species, thus making these strains generalist pathogens. This study reports the whole genome comparison of L. borgpetersenii serogroup Javanica strains isolated from Rattus species in East Asian states. The data herein suggest that the serogroup Javanica strains can infect the dominant Rattus species in each region and can be genetically diversified in a geographic manner.
钩端螺旋体属是钩端螺旋体病的病原体，该病是一种全球性的动物传染病，由各种哺乳动物维持。最近的分子分型研究表明，存在特定的钩端螺旋体基因型-宿主关联；然而，很少有研究表明单一基因型在多种动物属中定植。为了更深入地了解钩端螺旋体基因型-宿主关联，我们对从日本南部、菲律宾和中国台湾的不同 Rattus 物种中分离的 35 株 Javanica 血清群伯氏钩端螺旋体进行了全基因组测序。基于 SNP 的系统发育树显示，根据地理位置将菌株分为五个聚类，表明 Javanica 血清群伯氏钩端螺旋体的遗传多样性存在地理结构。此外，系统发育分析表明，Javanica 血清群伯氏钩端螺旋体可以感染各种动物物种，因此这些菌株是普遍的病原体。本研究报告了从东亚国家的 Rattus 物种中分离的 Javanica 血清群伯氏钩端螺旋体菌株的全基因组比较。这些数据表明，Javanica 血清群菌株可以感染每个地区的优势 Rattus 物种，并可以以地理方式在遗传上多样化。
