Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology, and Metabolism, Department of Metabolism, Reproduction, and Digestion, Imperial College London, London, United Kingdom.
Section of Cell Biology and Functional Genomics, Division of Diabetes, Endocrinology, and Metabolism, Department of Metabolism, Reproduction, and Digestion, Imperial College London, London, United Kingdom; Lee Kong Chian School of Medicine, Nan Yang Technological University, Singapore.
J Biol Chem. 2020 Jul 3;295(27):8912-8913. doi: 10.1074/jbc.H120.014586.
Insulin is stored in secretory granules to facilitate rapid release in response to rising glucose levels, but the mechanisms by which these granules are identified and prioritized for secretion remains unclear. Using a fluorescent timer and flow cytometry-assisted organelle sorting, Yau develop an elegant approach to assess insulin secretion as a function of granule age in pancreatic islet beta cells. Their findings supply quantitative evidence supporting the age-dependent release of different granule pools and confirm earlier models of preferential release of younger granules.
胰岛素储存在分泌颗粒中，以响应葡萄糖水平的升高而快速释放，但这些颗粒如何被识别和优先分泌的机制尚不清楚。Yau 使用荧光计时器和流式细胞术辅助细胞器分选，开发了一种评估胰岛β细胞中胰岛素分泌随颗粒年龄变化的优雅方法。他们的研究结果提供了定量证据，支持不同颗粒库的年龄依赖性释放，并证实了先前关于优先释放年轻颗粒的模型。
