Mondino B J, Sidikaro Y, Sumner H
Department of Ophthalmology and Jules Stein Eye Institute, UCLA School of Medicine 90024.
Invest Ophthalmol Vis Sci. 1988 Jul;29(7):1195-8.
The complement system is involved not only in host defense against infection but also in autoimmune tissue damage. Using a radioimmunoassay, we sought to measure levels of C3a, C4a and C5a (activated complement fragments with anaphylatoxin functions) in human vitreous humor from eyes with and without vitreal inflammation. Vitreous from 11 patients with clinical evidence of vitritis (Group 2) had significantly higher levels of protein, C3a and C4a and significantly higher ratios of these anaphylatoxins to protein than vitreous from 19 patients without clinical evidence of vitritis (Group 1). The finding that not only the absolute levels of anaphylatoxins but also the ratios of anaphylatoxins to protein were significantly higher in vitreous from Group 2 than vitreous from Group 1 or normal plasma suggested that complement activation was taking place in inflamed vitreous. Because of irreversible binding to leukocytes, C5a is difficult to measure and correlate with complement activation, and we were unable to detect it in any vitreous sample.
补体系统不仅参与宿主抗感染防御，还参与自身免疫性组织损伤。我们采用放射免疫分析法，试图测定有或无玻璃体炎症的人眼玻璃体液中C3a、C4a和C5a（具有过敏毒素功能的活化补体片段）的水平。11例有玻璃体炎临床证据的患者（第2组）的玻璃体，其蛋白、C3a和C4a水平显著高于19例无玻璃体炎临床证据的患者（第1组）的玻璃体，且这些过敏毒素与蛋白的比率也显著更高。第2组玻璃体中不仅过敏毒素的绝对水平，而且过敏毒素与蛋白的比率均显著高于第1组玻璃体或正常血浆，这一发现表明补体激活正在炎症玻璃体中发生。由于C5a与白细胞的结合是不可逆的，因此难以测量且难以与补体激活相关联，我们在任何玻璃体液样本中均未检测到它。
