Mondino B J, Sumner H
Invest Ophthalmol Vis Sci. 1986 Aug;27(8):1288-92.
Radioimmunoassay was used to measure levels of C3a, C4a, and C5a in aqueous humor from 13 normal eyes, 8 noninflamed eyes with a history of surgery or inflammation, and 14 eyes with anterior uveitis. The authors were unable to measure levels of C3a, C4a, or C5a in normal aqueous humor. In noninflamed aqueous humor from eyes with a history of surgery or inflammation, the authors were unable to measure levels of C4a or C5a, but were able to measure low levels of C3a in 3/8 patients. In aqueous humor from eyes with anterior uveitis, the authors were able to measure levels of C3a in all 14 patients, C4a in 9/14 patients, and C5a in 5/14 patients. Patients with severe anterior uveitis had higher levels of C3a than those with moderate anterior uveitis. The higher ratios of anaphylatoxin to protein levels in inflamed aqueous humor, when compared to normal plasma or noninflamed aqueous humor, suggested that complement was being activated by either the classical or alternative pathways in inflamed aqueous humor. Measurable levels of C3a without detectable C4a in five patients with anterior uveitis suggested alternative pathway activation of complement.
采用放射免疫分析法测定了13只正常眼、8只曾有手术或炎症史的非炎症眼以及14只前葡萄膜炎患眼中房水中C3a、C4a和C5a的水平。作者无法检测到正常房水中C3a、C4a或C5a的水平。在曾有手术或炎症史的非炎症眼的房水中，作者无法检测到C4a或C5a的水平，但在8例患者中有3例检测到了低水平的C3a。在前葡萄膜炎患眼中，作者在所有14例患者中检测到了C3a的水平，在9/14例患者中检测到了C4a的水平，在5/14例患者中检测到了C5a的水平。重度前葡萄膜炎患者的C3a水平高于中度前葡萄膜炎患者。与正常血浆或非炎症房水相比，炎症房水中过敏毒素与蛋白质水平的比值更高，这表明炎症房水中补体通过经典途径或替代途径被激活。5例前葡萄膜炎患者中可检测到C3a水平而未检测到C4a水平，提示补体替代途径激活。
