Mondino B J, Sidikaro Y, Mayer F J, Sumner H L
Uveitis Center, Jules Stein Eye Institute, University of California-Los Angeles School of Medicine 90024-1771.
Invest Ophthalmol Vis Sci. 1990 May;31(5):798-804.
We measured levels of protein, of complement-derived anaphylatoxins (C3a, C4a, and C5a), and of the lymphokines interleukin-2 and gamma interferon, in vitreous humor from 10 AIDS patients with vitritis and retinitis (group 1). We compared these measurements with levels in vitreous from 7 patients with vitritis but without AIDS (group 2), 10 patients with vitreous hemorrhages (group 3), and 20 patients with retinal detachments or epiretinal membranes without clinical evidence of vitreal inflammation (group 4). Vitreous humor from 10 AIDS patients had measurable levels of interleukin-2 in three of nine samples, gamma interferon in six of nine samples, C3a and C4a in all ten samples, and C5a in only one of ten samples. Vitreous humor from group 1 did not differ significantly from vitreous from group 2. On the other hand, vitreous from group 1 had significantly higher levels of gamma interferon, C3a, and C4a, and higher ratios of these anaphylatoxins to protein, in comparison to vitreous in groups 3 and 4. The results of this study suggest that vitreous humor from AIDS patients with retinitis contains activated complement and may contain interleukin-2 and gamma interferon. Viral retinitis is associated with the presence of lymphokines in vitreous humor. Additionally, anaphylatoxin and gamma interferon levels, but not interleukin-2 levels, correlate with vitreal inflammation. This is the first study to measure interleukin-2, gamma interferon, and C5a levels in human vitreous humor.
我们检测了10例患有玻璃体炎和视网膜炎的艾滋病患者（第1组）玻璃体液中的蛋白质、补体衍生过敏毒素（C3a、C4a和C5a）以及淋巴因子白细胞介素-2和γ干扰素的水平。我们将这些检测结果与7例患有玻璃体炎但无艾滋病的患者（第2组）、10例玻璃体积血患者（第3组）以及20例有视网膜脱离或视网膜前膜且无玻璃体炎症临床证据的患者（第4组）的玻璃体液水平进行了比较。10例艾滋病患者的玻璃体液中，9个样本中有3个样本可检测到白细胞介素-2水平，9个样本中有6个样本可检测到γ干扰素水平，所有10个样本中均可检测到C3a和C4a水平，10个样本中只有1个样本可检测到C5a水平。第1组的玻璃体液与第2组的玻璃体液无显著差异。另一方面，与第3组和第4组的玻璃体液相比，第1组的玻璃体液中γ干扰素、C3a和C4a水平显著更高，且这些过敏毒素与蛋白质的比率更高。本研究结果表明，患有视网膜炎的艾滋病患者的玻璃体液中含有活化的补体，可能还含有白细胞介素-2和γ干扰素。病毒性视网膜炎与玻璃体液中淋巴因子的存在有关。此外，过敏毒素和γ干扰素水平与玻璃体炎症相关，但白细胞介素-2水平与玻璃体炎症无关。这是首次检测人玻璃体液中白细胞介素-2、γ干扰素和C5a水平的研究。
