Chvapil M, Gaines J A, Gilman T
Department of Surgery, University of Arizona College of Medicine, Tucson 85724.
J Burn Care Rehabil. 1988 May-Jun;9(3):279-84. doi: 10.1097/00004630-198805000-00009.
A total of 80 partial-thickness wounds (4.4 cm2 size, 400 micron deep) was inflicted by electrokeratome in the dermal skin layer of four piglets, 15 kg body weight. The wounds were treated with gauze (control), lanolin cream (Lanolor or Lanolin with emulsifiers, Squibb) or with human epidermal growth factor (EGF) delivered in lanolin cream (10 micrograms EGF/mL cream). The treatment was applied every 12 hours for 12 to 120 hours after wounding. The reepithelization rate of the wound was determined by standardized morphometric method. In addition, we measured the thickness of the dermis and cell counts in the dermis. We found that most of the statistically significant enhancement of the epithelization rate, thickness of the dermis, and higher cell count in the dermis were attributed to the effect of lanolin cream alone. The additional significant enhancement of healing by EGF over that of lanolin alone was documented in one of our experiments, but was only marginal. In another experiment using another commercial formulation of lanolin, we found no difference between the effect of EGF and lanolin. Several hypotheses were suggested to explain the effect of the two tested lanolin cream formulations, which induced strong inflammatory reaction in the wound.
对4头体重15千克的仔猪，用电角膜刀在其真皮层造成总共80处厚度为400微米、面积为4.4平方厘米的部分厚度伤口。伤口分别用纱布（对照组）、羊毛脂乳膏（Lanolor或含乳化剂的羊毛脂，施贵宝公司生产）或含人表皮生长因子（EGF）的羊毛脂乳膏（每毫升乳膏含10微克EGF）进行处理。在伤口形成后每12小时处理一次，持续12至120小时。通过标准化形态测量方法测定伤口的再上皮化率。此外，我们还测量了真皮厚度和真皮中的细胞计数。我们发现，大多数在统计学上显著的上皮化率提高、真皮厚度增加以及真皮中细胞计数增多都归因于羊毛脂乳膏本身的作用。在我们的一项实验中，记录到EGF相对于单独使用羊毛脂而言对愈合有额外的显著促进作用，但只是微不足道的。在另一项使用另一种市售羊毛脂制剂的实验中，我们发现EGF和羊毛脂的效果没有差异。有人提出了几个假说来解释两种受试羊毛脂乳膏制剂的作用，这两种制剂在伤口处引发了强烈的炎症反应。
