Department of Neuroscience - Psychiatry, Iuliu Hatieganu University of Medicine and Pharmacy, 43 Victor Babes Street, Cluj-Napoca, Romania.
Department of Physiology, Iuliu Hatieganu University of Medicine and Pharmacy, 1-3 Clinicilor Street, Cluj-Napoca, Romania.
J Mol Neurosci. 2020 Dec;70(12):1943-1961. doi: 10.1007/s12031-020-01593-w. Epub 2020 Jul 3.
The study investigated the potential neuroprotective effects of metformin (MET) on alcohol-induced neurotoxicity in adult Wistar rats. The animals were randomized in four groups (n = 10): control, alcohol (ALC), ALC + MET, and MET. ALC (2 g/kg b.w.) and MET (200 mg/kg b.w.) were orally administered for 21 days, once daily. For the ALC + MET group, MET was administered 2 h after ALC treatment. On day 22, the open field test (OFT) and elevated plus maze (EPM) were performed. MET improved global activity and increased the time spent in unprotected open arms, decreased oxidative stress, both in the frontal lobe and in the hippocampus, and increased neuroglobin expression in the frontal cortex. Histopathologically, an increased neurosecretory activity in the frontal cortex in the ALC + MET group was noticed. Thus, our findings suggest that metformin has antioxidant and anxiolytic effects and may partially reverse the neurotoxic effects induced by ethanol.
该研究调查了二甲双胍（MET）对成年 Wistar 大鼠酒精诱导的神经毒性的潜在神经保护作用。动物随机分为四组（n=10）：对照组、酒精组（ALC）、酒精+MET 组和 MET 组。ALC（2 g/kg b.w.）和 MET（200 mg/kg b.w.）每天口服一次，连续 21 天。对于 ALC+MET 组，MET 在 ALC 处理后 2 小时给予。第 22 天，进行旷场试验（OFT）和高架十字迷宫试验（EPM）。MET 改善了整体活动，增加了无保护的开放臂停留时间，减少了前额叶和海马中的氧化应激，并增加了前额皮质中的神经球蛋白表达。组织病理学上，在 ALC+MET 组中注意到前额皮质的神经分泌活性增加。因此，我们的研究结果表明，二甲双胍具有抗氧化和抗焦虑作用，并可能部分逆转乙醇诱导的神经毒性。
