Jacobs S C, Story M T
Department of Urology, Medical College of Wisconsin, Milwaukee.
Prostate. 1988;13(1):79-87. doi: 10.1002/pros.2990130109.
Perfusion of the rat prostatic urethra in vivo provided a means of collecting the rat prostatic secretory product. The secretion of the protein epidermal growth factor (EGF) was investigated by radioimmunoassay (RIA). Baseline secretion of EGF into prostatic fluid was less than .03 +/- .004 SEM ng/min. The alpha-adrenergic agonist phenylephrine caused an increase in EGF to 3.6 +/- .4 SEM ng per 30-min period. The stimulation was blocked completely by prazosin and only partially by yohimbine, indicating primarily alpha 1 control. One mg/kg IV phenylephrine produced a maximal response. The beta-adrenergic agonist isoproterenol caused no increased secretion of EGF. The cholinergic agonist pilocarpine stimulated EGF secretion to 3.2 +/- .6 SEM ng per 30-min period. Atropine blocked the cholinergic stimulation. The combination of phenylephrine and pilocarpine did not result in greater stimulation than either agent alone. Vasoactive intestinal peptide (VIP) did not stimulate EGF secretion, nor did it augment either pilocarpine or phenylephrine-stimulated secretion. EGF secretion into rat prostatic fluid is under both alpha 1-adrenergic and cholinergic control.
对大鼠前列腺尿道进行体内灌注提供了一种收集大鼠前列腺分泌产物的方法。通过放射免疫测定法（RIA）研究了蛋白质表皮生长因子（EGF）的分泌情况。前列腺液中EGF的基础分泌量低于0.03±0.004 SEM纳克/分钟。α-肾上腺素能激动剂去氧肾上腺素使EGF在每30分钟内增加至3.6±0.4 SEM纳克。哌唑嗪可完全阻断该刺激作用，而育亨宾仅部分阻断，表明主要受α1控制。静脉注射1毫克/千克去氧肾上腺素可产生最大反应。β-肾上腺素能激动剂异丙肾上腺素未引起EGF分泌增加。胆碱能激动剂毛果芸香碱将EGF分泌刺激至每30分钟3.2±0.6 SEM纳克。阿托品可阻断胆碱能刺激。去氧肾上腺素和毛果芸香碱联合使用并未比单独使用任何一种药物产生更大的刺激作用。血管活性肠肽（VIP）未刺激EGF分泌，也未增强毛果芸香碱或去氧肾上腺素刺激的分泌。大鼠前列腺液中EGF的分泌受α1-肾上腺素能和胆碱能的双重控制。
